LOCUS       sequence-113860-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to a 3xT7 
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pFvl160
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(131..163)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(172..205)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        300..643
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aaattaaccc tcactaaagg gaacaaaagc ttgcatgcgc ggccgcttat gtacatccca
       61 tttgctggcc accggtcatt gaggccattc ctcccatctg ttgacctcct gtcatagaag
      121 ccatagtaca acccatttgc tgtccaccag tcatgcttgc catgtcgact gataacttcg
      181 tatagcatac attatacgaa gttattctag actgtgcggt atttcacacc gcatagggtt
      241 aattaaggcg cgccagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg
      301 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
      361 gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac
      421 attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg acctgcgagc
      481 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
      541 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
      601 tgctaggata cagttctcac atcacatccg aacataaaca accatgggta aaaagcctga
      661 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
      721 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
      781 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
      841 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
      901 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
      961 aaccgaactg cccgctgttc tgcagccggt cgcgg
//
