LOCUS       sequence-113863-       12202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 safe harbor gene targeting donor expressing CAG-driven 
            Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 12202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pAAVS1P-iCLHN
FEATURES             Location/Qualifiers
     source          1..12202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             114..923
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(181..200)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1101..1689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1590..1609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1843..1860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1977..1998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2013..2018,2019..2036,2037..2043)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2051..2067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2056..2078
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2075..2091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2075..2091
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    2112..2915
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(2924..2957)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2958..2983
                     /label=SA
                     /note="splice acceptor site"
     CDS             3007..3060
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3064..3663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3064..3083)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3560..3580
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3664..3888
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3889..3922)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        5174..5553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5555..5832
                     /label=chicken beta-actin promoter
     intron          5833..6850
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6774..6796
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6858..6877
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6902..7414
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             7433..8317
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             8318..8377
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             8378..9169
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8429..8448)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9039..9058
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9259..9278)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9324..9379
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9378..9397)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    10963..11799
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(11808..11825)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11808..11824)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11817..11839)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12033..12052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12152..12174
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
       61 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      121 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      181 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      241 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      301 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      361 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      421 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      481 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      541 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      601 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
      661 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      721 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
      781 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
      841 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
      901 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt
      961 gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2041 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     2101 gccgcggccg ctgctttctc tgaccagcat tctctcccct gggcctgtgc cgctttctgt
     2161 ctgcagcttg tggcctgggt cacctctacg gctggcccag atccttccct gccgcctcct
     2221 tcaggttccg tcttcctcca ctccctcttc cccttgctct ctgctgtgtt gctgcccaag
     2281 gatgctcttt ccggagcact tccttctcgg cgctgcacca cgtgatgtcc tctgagcgga
     2341 tcctccccgt gtctgggtcc tctccgggca tctctcctcc ctcacccaac cccatgccgt
     2401 cttcactcgc tgggttccct tttccttctc cttctggggc ctgtgccatc tctcgtttct
     2461 taggatggcc ttctccgacg gatgtctccc ttgcgtcccg cctccccttc ttgtaggcct
     2521 gcatcatcac cgtttttctg gacaacccca aagtaccccg tctccctggc tttagccacc
     2581 tctccatcct cttgctttct ttgcctggac accccgttct cctgtggatt cgggtcacct
     2641 ctcactcctt tcatttgggc agctccccta ccccccttac ctctctagtc tgtgctagct
     2701 cttccagccc cctgtcatgg catcttccag gggtccgaga gctcagctag tcttcttcct
     2761 ccaacccggg cccctatgtc cacttcagga cagcatgttt gctgcctcca gggatcctgt
     2821 gtccccgagc tgggaccacc ttatattccc agggccggtt aatgtggctc tggttctggg
     2881 tacttttatc tgtcccctcc accccacagt ggggcgcggc cgcataactt cgtatagcat
     2941 acattatacg aagttatctg acctcttctc ttcctcccac agggcctcga gagatctggc
     3001 agcggagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     3061 aggatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     3121 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     3181 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     3241 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     3301 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     3361 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     3421 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     3481 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     3541 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     3601 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     3661 tgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3721 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3781 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3841 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggat aacttcgtat
     3901 agcatacatt atacgaagtt atgagctcac ggggacagcc cccccccaaa gcccccaggg
     3961 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     4021 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     4081 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     4141 cctgggggga tacggggaaa aagctttagg ctgaaagaga gatttagaat gacagaatca
     4201 tagaacggcc tgggttgcaa aggagcacag tgctcatcca gatccaaccc cctgctatgt
     4261 gcagggtcat caaccagcag cccaggctgc ccagagccac atccagcctg gccttgaatg
     4321 cctgcaggga tggggcatcc acagcctcct tgggcaacct gttcagtgcg tcaccaccct
     4381 ctgggggaaa aactgcctcc tcatatccaa cccaaacctc ccctgtctca gtgtaaagcc
     4441 attccccctt gtcctatcaa gggggagttt gctgtgacat tgttggtctg gggtgacaca
     4501 tgtttgccaa ttcagtgcat cacggagagg cagatcttgg ggataaggaa gtgcaggaca
     4561 gcatggacgt gggacatgca ggtgttgagg gctctgggac actctccaag tcacagcgtt
     4621 cagaacagcc ttaaggataa gaagatagga tagaaggaca aagagcaagt taaaacccag
     4681 catggagagg agcacaaaaa ggccacagac actgctggtc cctgtgtctg agcctgcatg
     4741 tttgatggtg tctggatgca agcagaaggg gtggaagagc ttgcctggag agatacagct
     4801 gggtcagtag gactgggaca ggcagctgga gaattgccat gtagatgttc atacaatcgt
     4861 caaatcatga aggctggaaa agccctccaa gatccccaag accaacccca acccacccac
     4921 cgtgcccact ggccatgtcc ctcagtgcca catccccaca gttcttcatc acctccaggg
     4981 acggtgaccc ccccacctcc gtgggcagct gtgccactgc agcaccgctc tttggagaag
     5041 gtaaatcttg ctaaatccag cccgaccctc ccctggcaca acgtaaggcc attatctctc
     5101 atccaactcc aggacggagt cagtgagaat attggcgcgc cgaattctcg cgacgatcgg
     5161 tcgacattta aatgacattg attattgact agttattaat agtaatcaat tacggggtca
     5221 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     5281 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     5341 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     5401 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     5461 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     5521 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
     5581 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     5641 ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg
     5701 ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg
     5761 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     5821 gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc
     5881 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
     5941 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
     6001 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     6061 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
     6121 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
     6181 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     6241 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     6301 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     6361 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     6421 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc
     6481 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     6541 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     6601 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     6661 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     6721 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     6781 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     6841 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     6901 aatggtcttc acactcgaag atttcgttgg ggactggcga cagacagccg gctacaacct
     6961 ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt
     7021 aactccgatc caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt
     7081 catcatcccg tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa
     7141 ggtggtgtac cctgtggatg atcatcactt taaggtgatc ctgcactatg gcacactggt
     7201 aatcgacggg gttacgccga acatgatcga ctatttcgga cggccgtatg aaggcatcgc
     7261 cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg tggaacggca acaaaattat
     7321 cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc cgagtaacca tcaacggagt
     7381 gaccggctgg cggctgtgcg aacgcattct ggcgggctcg agcggcggat ccgaaatcgg
     7441 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt
     7501 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc
     7561 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga
     7621 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt
     7681 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca
     7741 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg
     7801 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc
     7861 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca
     7921 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga
     7981 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt
     8041 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata
     8101 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt
     8161 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt
     8221 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga
     8281 gatcgcgcgc tggctgtcta ctctggagat ttccggtcaa tgtactaact acgctttgtt
     8341 gaaactcgct ggcgatgttg aaagtaaccc cggtcctatt gaacaagatg gattgcacgc
     8401 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     8461 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8521 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     8581 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     8641 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     8701 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     8761 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     8821 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     8881 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     8941 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     9001 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     9061 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9121 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctaaa cgcgtgaatt
     9181 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     9241 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     9301 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     9361 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     9421 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     9481 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     9541 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     9601 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     9661 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatcgatat cggtacctta
     9721 attaagttaa catgcatagt actgagctca cggggacagc ccccccccaa agcccccagg
     9781 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
     9841 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
     9901 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
     9961 acctgggggg atacggggaa aaagctttag gctgaaagag agatttagaa tgacagaatc
    10021 atagaacggc ctgggttgca aaggagcaca gtgctcatcc agatccaacc ccctgctatg
    10081 tgcagggtca tcaaccagca gcccaggctg cccagagcca catccagcct ggccttgaat
    10141 gcctgcaggg atggggcatc cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc
    10201 tctgggggaa aaactgcctc ctcatatcca acccaaacct cccctgtctc agtgtaaagc
    10261 cattccccct tgtcctatca agggggagtt tgctgtgaca ttgttggtct ggggtgacac
    10321 atgtttgcca attcagtgca tcacggagag gcagatcttg gggataagga agtgcaggac
    10381 agcatggacg tgggacatgc aggtgttgag ggctctggga cactctccaa gtcacagcgt
    10441 tcagaacagc cttaaggata agaagatagg atagaaggac aaagagcaag ttaaaaccca
    10501 gcatggagag gagcacaaaa aggccacaga cactgctggt ccctgtgtct gagcctgcat
    10561 gtttgatggt gtctggatgc aagcagaagg ggtggaagag cttgcctgga gagatacagc
    10621 tgggtcagta ggactgggac aggcagctgg agaattgcca tgtagatgtt catacaatcg
    10681 tcaaatcatg aaggctggaa aagccctcca agatccccaa gaccaacccc aacccaccca
    10741 ccgtgcccac tggccatgtc cctcagtgcc acatccccac agttcttcat cacctccagg
    10801 gacggtgacc cccccacctc cgtgggcagc tgtgccactg cagcaccgct ctttggagaa
    10861 ggtaaatctt gctaaatcca gcccgaccct cccctggcac aacgtaaggc cattatctct
    10921 catccaactc caggacggag tcagtgagaa tattgtttaa acactaggga caggattggt
    10981 gacagaaaag ccccatcctt aggcctcctc cttcctagtc tcctgatatt gggtctaacc
    11041 cccacctcct gttaggcaga ttccttatct ggtgacacac ccccatttcc tggagccatc
    11101 tctctccttg ccagaacctc taaggtttgc ttacgatgga gccagagagg atcctgggag
    11161 ggagagcttg gcagggggtg ggagggaagg gggggatgcg tgacctgccc ggttctcagt
    11221 ggccaccctg cgctaccctc tcccagaacc tgagctgctc tgacgcggct gtctggtgcg
    11281 tttcactgat cctggtgctg cagcttcctt acacttccca agaggagaag cagtttggaa
    11341 aaacaaaatc agaataagtt ggtcctgagt tctaactttg gctcttcacc tttctagtcc
    11401 ccaatttata ttgttcctcc gtgcgtcagt tttacctgtg agataaggcc agtagccagc
    11461 cccgtcctgg cagggctgtg gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga
    11521 gaatggtgcg tcctaggtgt tcaccaggtc gtggccgcct ctactccctt tctctttctc
    11581 catccttctt tccttaaaga gtccccagtg ctatctggga catattcctc cgcccagagc
    11641 agggtcccgc ttccctaagg ccctgctctg ggcttctggg tttgagtcct tggcaagccc
    11701 aggagaggcg ctcaggcttc cctgtccccc ttcctcgtcc accatctcat gcccctggct
    11761 ctcctgcccc ttccctacag gggttcctgg ctctgctctg tttaaacact ggccgtcgtt
    11821 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    11881 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    11941 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
    12001 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    12061 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
    12121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
    12181 ccgtcatcac cgaaacgcgc ga
//
