LOCUS       sequence-113864-       12189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CLYBL safe harbor gene targeting donor expressing CAG-driven 
            Nanoluc-HaloTag, compatible with pZT-C13 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 12189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pC13P-iCLHN
FEATURES             Location/Qualifiers
     source          1..12189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             114..923
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(181..200)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1101..1689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1590..1609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1843..1860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1977..1998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2013..2018,2019..2036,2037..2043)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2051..2067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2056..2078
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2075..2091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2075..2091
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(2952..2985)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2986..3011
                     /label=SA
                     /note="splice acceptor site"
     CDS             3035..3088
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3092..3691
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3092..3111)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3588..3608
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3692..3916
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3917..3950)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        5202..5581
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5583..5860
                     /label=chicken beta-actin promoter
     intron          5861..6878
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6802..6824
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6886..6905
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6930..7442
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             7461..8345
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             8346..8405
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             8406..9197
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8457..8476)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9067..9086
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9287..9306)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9352..9407
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9406..9425)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11795..11812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11795..11811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11804..11826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12020..12039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12139..12161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
       61 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      121 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      181 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      241 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      301 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      361 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      421 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      481 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      541 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      601 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
      661 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      721 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
      781 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
      841 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
      901 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt
      961 gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2041 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     2101 gccgcggccg cagcaggaga atcatgccaa tgggccaata tacattctga cccacagttt
     2161 cataataaaa taaaatggtt gtggttgtaa gccactatgt ttcagagtgg tttgttacac
     2221 agcaataaat aactaatata gtaggcatac catcaagtcc aaagtaggta gagaagaatg
     2281 taaatagcag agcaaaacag catgactggt ggctgggagg cttaaaactg ggacaggatc
     2341 agagtcatga aagaagtcaa agaaatggtt cagaagtaag gctgagactg acttacaaaa
     2401 gctgaaagtc cctttaagtt ggtgtttggt gcattggcag gggcaggtat ggtgacttaa
     2461 aagagccatg ctcaacaaga tcaagcacaa cacaatcacg ggtcacccca gcagacctta
     2521 gcgagtctag ccatttcttt ggtggtggtc acagtcatgc ttcagcccag tttccacttg
     2581 gacaaatggt acatattttc aatgagatga aaattaagat acaatccatg tgctcagaga
     2641 gtgatcacag ctctgactaa acactgtgcc ccaaagtgtt gaggaattgg gaaaacctag
     2701 ctgagttagt ggtctctttt ctgttacaat aaagctcata atgaaaatta gccttctttg
     2761 ttcttcccca agtctttctt tctagacgaa actactttca actgttttaa cttccttact
     2821 gttaacttcc atatttctag atagtatggt cagactgtta tttcttgact tttaaatgta
     2881 agatattatc tactgacttc cttctatgta agatgaggat tgagctctct tacccttctc
     2941 ccagcggccg cataacttcg tatagcatac attatacgaa gttatctgac ctcttctctt
     3001 cctcccacag ggcctcgaga gatctggcag cggagagggc agaggaagtc ttctaacatg
     3061 cggtgacgtg gaggagaatc ccggccctag gatgaccgag tacaagccca cggtgcgcct
     3121 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     3181 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     3241 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     3301 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     3361 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     3421 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     3481 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     3541 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     3601 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     3661 ctggtgcatg acccgcaagc ccggtgcctg actgtgcctt ctagttgcca gccatctgtt
     3721 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3781 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3841 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3901 gcggtgggct ctatggataa cttcgtatag catacattat acgaagttat gagctcacgg
     3961 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca
     4021 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc
     4081 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc
     4141 ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct
     4201 gaaagagaga tttagaatga cagaatcata gaacggcctg ggttgcaaag gagcacagtg
     4261 ctcatccaga tccaaccccc tgctatgtgc agggtcatca accagcagcc caggctgccc
     4321 agagccacat ccagcctggc cttgaatgcc tgcagggatg gggcatccac agcctccttg
     4381 ggcaacctgt tcagtgcgtc accaccctct gggggaaaaa ctgcctcctc atatccaacc
     4441 caaacctccc ctgtctcagt gtaaagccat tcccccttgt cctatcaagg gggagtttgc
     4501 tgtgacattg ttggtctggg gtgacacatg tttgccaatt cagtgcatca cggagaggca
     4561 gatcttgggg ataaggaagt gcaggacagc atggacgtgg gacatgcagg tgttgagggc
     4621 tctgggacac tctccaagtc acagcgttca gaacagcctt aaggataaga agataggata
     4681 gaaggacaaa gagcaagtta aaacccagca tggagaggag cacaaaaagg ccacagacac
     4741 tgctggtccc tgtgtctgag cctgcatgtt tgatggtgtc tggatgcaag cagaaggggt
     4801 ggaagagctt gcctggagag atacagctgg gtcagtagga ctgggacagg cagctggaga
     4861 attgccatgt agatgttcat acaatcgtca aatcatgaag gctggaaaag ccctccaaga
     4921 tccccaagac caaccccaac ccacccaccg tgcccactgg ccatgtccct cagtgccaca
     4981 tccccacagt tcttcatcac ctccagggac ggtgaccccc ccacctccgt gggcagctgt
     5041 gccactgcag caccgctctt tggagaaggt aaatcttgct aaatccagcc cgaccctccc
     5101 ctggcacaac gtaaggccat tatctctcat ccaactccag gacggagtca gtgagaatat
     5161 tggcgcgccg aattctcgcg acgatcggtc gacatttaaa tgacattgat tattgactag
     5221 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     5281 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     5341 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     5401 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     5461 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     5521 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     5581 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     5641 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     5701 ggggcccccc ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg
     5761 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     5821 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct
     5881 tccccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     5941 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     6001 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
     6061 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
     6121 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     6181 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     6241 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
     6301 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga
     6361 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc
     6421 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     6481 gggctcgggg gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     6541 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     6601 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     6661 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     6721 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     6781 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     6841 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     6901 ttgtgctgtc tcatcatttt ggcaaagaaa tggtcttcac actcgaagat ttcgttgggg
     6961 actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga ggtgtgtcca
     7021 gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg
     7081 aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg agcggcgacc
     7141 aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat catcacttta
     7201 aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac atgatcgact
     7261 atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc actgtaacag
     7321 ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc gacggctccc
     7381 tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa cgcattctgg
     7441 cgggctcgag cggcggatcc gaaatcggta ctggctttcc attcgacccc cattatgtgg
     7501 aagtcctggg cgagcgcatg cactacgtcg atgttggtcc gcgcgatggc acccctgtgc
     7561 tgttcctgca cggtaacccg acctcctcct acgtgtggcg caacatcatc ccgcatgttg
     7621 caccgaccca tcgctgcatt gctccagacc tgatcggtat gggcaaatcc gacaaaccag
     7681 acctgggtta tttcttcgac gaccacgtcc gcttcatgga tgccttcatc gaagccctgg
     7741 gtctggaaga ggtcgtcctg gtcattcacg actggggctc cgctctgggt ttccactggg
     7801 ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat ggagttcatc cgccctatcc
     7861 cgacctggga cgaatggcca gaatttgccc gcgagacctt ccaggccttc cgcaccaccg
     7921 acgtcggccg caagctgatc atcgatcaga acgtttttat cgagggtacg ctgccgatgg
     7981 gtgtcgtccg cccgctgact gaagtcgaga tggaccatta ccgcgagccg ttcctgaatc
     8041 ctgttgaccg cgagccactg tggcgcttcc caaacgagct gccaatcgcc ggtgagccag
     8101 cgaacatcgt cgcgctggtc gaagaataca tggactggct gcaccagtcc cctgtcccga
     8161 agctgctgtt ctggggcacc ccaggcgttc tgatcccacc ggccgaagcc gctcgcctgg
     8221 ccaaaagcct gcctaactgc aaggctgtgg acatcggccc gggtctgaat ctgctgcaag
     8281 aagacaaccc ggacctgatc ggcagcgaga tcgcgcgctg gctgtctact ctggagattt
     8341 ccggtcaatg tactaactac gctttgttga aactcgctgg cgatgttgaa agtaaccccg
     8401 gtcctattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     8461 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     8521 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     8581 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     8641 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     8701 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     8761 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     8821 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     8881 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     8941 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     9001 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     9061 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     9121 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     9181 ttgacgagtt cttctaaacg cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg
     9241 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     9301 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     9361 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     9421 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     9481 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     9541 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     9601 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     9661 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     9721 tcttatggag atcgatatcg gtaccttaat taagttaaca tgcatagtac tgagctcacg
     9781 gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc
     9841 agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg
     9901 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg
     9961 cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa agctttaggc
    10021 tgaaagagag atttagaatg acagaatcat agaacggcct gggttgcaaa ggagcacagt
    10081 gctcatccag atccaacccc ctgctatgtg cagggtcatc aaccagcagc ccaggctgcc
    10141 cagagccaca tccagcctgg ccttgaatgc ctgcagggat ggggcatcca cagcctcctt
    10201 gggcaacctg ttcagtgcgt caccaccctc tgggggaaaa actgcctcct catatccaac
    10261 ccaaacctcc cctgtctcag tgtaaagcca ttcccccttg tcctatcaag ggggagtttg
    10321 ctgtgacatt gttggtctgg ggtgacacat gtttgccaat tcagtgcatc acggagaggc
    10381 agatcttggg gataaggaag tgcaggacag catggacgtg ggacatgcag gtgttgaggg
    10441 ctctgggaca ctctccaagt cacagcgttc agaacagcct taaggataag aagataggat
    10501 agaaggacaa agagcaagtt aaaacccagc atggagagga gcacaaaaag gccacagaca
    10561 ctgctggtcc ctgtgtctga gcctgcatgt ttgatggtgt ctggatgcaa gcagaagggg
    10621 tggaagagct tgcctggaga gatacagctg ggtcagtagg actgggacag gcagctggag
    10681 aattgccatg tagatgttca tacaatcgtc aaatcatgaa ggctggaaaa gccctccaag
    10741 atccccaaga ccaaccccaa cccacccacc gtgcccactg gccatgtccc tcagtgccac
    10801 atccccacag ttcttcatca cctccaggga cggtgacccc cccacctccg tgggcagctg
    10861 tgccactgca gcaccgctct ttggagaagg taaatcttgc taaatccagc ccgaccctcc
    10921 cctggcacaa cgtaaggcca ttatctctca tccaactcca ggacggagtc agtgagaata
    10981 ttgtttaaac tcctcatcct tccaacataa atatattttg ggattatatc aacattcaat
    11041 gttacttaaa gtgaccttgt aaatattttc acaactgagc catgtttgat ttgtatactt
    11101 atgtttactt tactgttttt cctgaagtta ataattgcct tgaatttatt tatttcttta
    11161 aaaatgtttc attactcagg actgtagttt acattacgat tctttgtgtt atacagttga
    11221 tgggtttctt ttctttctta atttctttaa aaaatagaga tggggtctta ctatattacc
    11281 caggctggtc ttgaagtcct gggctcaagt gatcttcctg tctcagccta ccaagtagct
    11341 gagactatag gtgcaaaaaa gccactatac ctggctagtt tacaggtttt aacaaatgca
    11401 ttatgccacg tatccattat tacaggatca cacaagatat tttcattacc ctgaaagcat
    11461 ccctgtgttc caccaattca tcctgcctcc atgagccgct ggcaaccact gatctctata
    11521 gttttgcctt ttctaaaatg tcatataatt ggaatcatac agtctgtagc attttcagac
    11581 tagcttttaa aatttggcaa tatgcattta aggttcctcc ttaaatgtga agggcatagc
    11641 caatgtggct tgatagctca tttcttttta ttggtgaata tttcattgtc tggatgttcc
    11701 acagtttgtt tatccattca cctattcaat ttgctttttt tctgtgtatc tatcactaat
    11761 tcaatactgg actctccaac agagccgttt aaacactggc cgtcgtttta caacgtcgtg
    11821 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
    11881 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
    11941 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
    12001 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
    12061 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
    12121 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
    12181 aacgcgcga
//
