LOCUS       sequence-113869-        3775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Specific and conditional cell ablation of beta cells in the 
            zebrafish pancreas.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Curado S, Anderson RM, Jungblut B, Mumm J, Schroeter E, Stainier DY
  TITLE     Conditional targeted cell ablation in zebrafish: a new tool for 
            regeneration studies.
  JOURNAL   Dev Dyn. 2007 Apr . 236(4):1025-35.
  PUBMED    17326133
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Apr . 236(4):1025-35."
COMMENT     Sequence Label: ins:CFP-NTR
FEATURES             Location/Qualifiers
     source          1..3775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..63
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    181..202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(217..222,223..240,241..247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    255..271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     260..282
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     279..295
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     279..295
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     341..360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        341..359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1540..1542,1543..1545,1546..2256)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1585..1606)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1846..1865)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2193..2214
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3220..3341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3257..3276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3311..3330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3474..3494)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3474..3492)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3525..3542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3525..3541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3534..3556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
       61 gaggaagcgg aagagcgccc aatacgcaaa ccgccttntc cccgcgcgtt ggccgattca
      121 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      181 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      241 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      301 ttacgccaag cgcgttaggg ataacagggt aatgcgcgcg taatacgact cactataggg
      361 cgaattggag ctcacgcgta agctttgttt gtaccataat aagctcatta tataatattt
      421 aaaatgatac tgcatcatta attgatatgt tttctagtta tttttaaata ttagcaacta
      481 aattaggaaa ttatccatag taaatttaat gtaatattat ttgttatatt taacttcagc
      541 ccacagtcta gtttagttgt tggtccctta tgagaaaaaa tttgggcacc tctgctctag
      601 tgcttgtaaa catttttgag tatctatagt tgaacatgaa agcatttttt tttttttttt
      661 tttaagaaaa tgaagaaagc taaaaacctg tatccactga cttccattaa aaaaagcaaa
      721 tactagggat gtctatggtg tcttcttttg tgttcaacaa ataagcagaa agtaactgct
      781 gacagaacat tacattttgt gtgaatcccc ttatattgtg ttaaaaactg tagagaaatc
      841 atctaataat aagaaattat caagaaataa acagaaaatg atctaatagt atactataaa
      901 atctttcaaa atccaggcca ctaccatatg actaaaacca cacatattat gctttaaaaa
      961 aagtcacaat gaagacgtca caaacgttcc tgaagactaa tgactcgtct aaccccatct
     1021 taccttctgc tgtttcactc aaagaaaagc caccagctgt ctctgtgatc tgctcattct
     1081 gtgttaactt gtgttctcaa caattaacaa ttaaccaaaa gcctctggag ctgcgtgagc
     1141 atcacgatgt gtttgtcttc tgtgagcgtg gcagtgtcca cgaagtcaat gtgaattggt
     1201 tggccaaaca cagctaatgt ggcatgtgtg attggacagc cacttaaaag tgttaaagtg
     1261 tgtttacgca caggattggt tttccgctga atcaggcaaa accagcagaa ccgatctaaa
     1321 ctcgagtata aaagtgagta ggagatgcta ttcgtcccaa aacatctcca ccaccatctc
     1381 caccattcct cgcctctgct tcgagaacag gtgagtgtcg agcgggatgg taaatctaca
     1441 gagaatgcgg agtgtagctt gtgtacatgt ttttgattaa cagagattgt atgtgtgtgt
     1501 ttgtgtcagt gtgacggatc ccccgggctg caggaattca tggtgagcaa gggcgaggag
     1561 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1621 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1681 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
     1741 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1801 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1861 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1921 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
     1981 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     2041 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2101 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2161 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2221 gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagccatg
     2281 gatatcattt ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa
     2341 cttaccccgg aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc
     2401 aactcccagc cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc
     2461 aaatccgctg ccggtaatta cgtgttcaac gagcgtaaaa tgcttgatgc ctcgcacgtc
     2521 gtggtgttct gtgcaaaaac cgcgatggac gatgtctggc tgaagctggt tgttgaccag
     2581 gaagatgccg atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc
     2641 aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa
     2701 caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg cggctctggg tctggacgcg
     2761 gtacccatcg aaggttttga cgccgccatc ctcgatgcag aatttggtct gaaagagaaa
     2821 ggctacacca gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct
     2881 acgctgccga aatctcgtct gcagcaaaac atcaccttaa ccgaagtgta attctctctt
     2941 gccgggcatc tgcccggcta tttcctctca gattctcctg atttgcataa ccctgtttca
     3001 gccgtcatca taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg
     3061 ttgaagattt agcgggtatt gagatcgatc ggatccaccg gatctagata actgatcata
     3121 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3181 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3241 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3301 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     3361 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3421 ggccgaaatc ggcagtcgac ctcgaggggg ggcccggtac ccagcttttg ttccctttag
     3481 tgagggttaa ttgcgcgcta gggataacag ggtaatacgc gctcactggc cgtcgtttta
     3541 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3601 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3661 cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3721 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atccc
//
