LOCUS       sequence-113879-        9760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses lambdaN-HA-tagged EDC4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Tritschler F, Braun JE, Motz C, Igreja C, Haas G, Truffault V, 
            Izaurralde E, Weichenrieder O
  TITLE     DCP1 forms asymmetric trimers to assemble into active mRNA decapping
            complexes in metazoa.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6.
  PUBMED    19966221
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Dec 22. 106(51):21591-6."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsEDC4
FEATURES             Location/Qualifiers
     source          1..9760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1185..1211
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    5464..5585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5475..5494)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5529..5548
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5771..6226
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5858..5877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6068..6089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6343..6700
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6343..6534
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6366..6386)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6551..6686
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6751..7545
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6805..6824)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7415..7434
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7609..7657
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7698..7717)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7817..7839
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7877..7895)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7963..8067
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8068..8136,8137..8928)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8286..8305)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9099..9687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9588..9607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agaattcaat ggcttaccca tacgatgttc
     1201 cagattacgc tatggcctcc tgcgcgagca tcgacatcga ggacgccacg cagcacctgc
     1261 gggacatcct caagctggac cggcccgcgg gcggccccag tgcagagagc ccacggccat
     1321 ccagtgccta caatggggac ctcaatggac ttctggtccc agacccgctc tgctcaggtg
     1381 atagtacctc agcaaacaag actggtcttc ggaccatgcc acccattaac ctgcaagaga
     1441 agcaggtcat ctgtctctca ggagatgata gctccacctg cattgggatt ttggccaagg
     1501 aggtggagat tgtggctagc agtgactcta gcatttcaag caaggcccgg ggaagcaaca
     1561 aggtgaaaat tcagcctgtc gccaagtatg actgggaaca gaagtactac tatggcaacc
     1621 tgattgctgt gtctaactcc ttcttggcct atgccattcg ggctgccaac aatggctctg
     1681 ccatggtgcg ggtgatcagc gtcagcactt cggagcggac cttgctcaag ggcttcacag
     1741 gcagtgtggc tgatctggct ttcgcgcacc tcaactctcc acagctggcc tgcctggatg
     1801 aggcaggcaa cctgttcgtg tggcgcttgg ctctggttaa tggcaaaatt caagaagaga
     1861 tcttggtcca tattcggcag ccagagggca cgccactgaa ccactttcgc aggatcatct
     1921 ggtgcccctt catccctgag gagagcgaag actgctgtga ggagagcagc ccaacagtgg
     1981 ccctgctgca tgaagaccgg gctgaggtgt gggacctgga catgctccgc tccagccaca
     2041 gtacctggcc tgtggatgtt agccagatca agcagggctt cattgtggta aaaggtcata
     2101 gcacgtgcct cagtgaagga gccctctctc ctgatgggac tgtgctggct actgcgagcc
     2161 acgatggcta tgtcaagttc tggcagatct acattgaggg gcaagatgag ccaaggtgtc
     2221 tgcacgagtg gaaacctcat gatgggcggc ccctctcctg cctcctgttc tgtgacaacc
     2281 ataagaaaca agaccctgat gtccctttct ggaggttcct tattactggt gctgaccaga
     2341 accgagagtt aaagatgtgg tgtacagtat cctggacctg cctgcagact attcgcttct
     2401 ccccagatat cttcagctca gtgagtgtgc cccctagcct caaggtttgc ttggacctct
     2461 cagcagaata cctgattctc agcgatgtgc aacggaaggt cctctatgtg atggagctgc
     2521 tgcaaaacca ggaggagggc cacgcctgct tcagctccat ctcggagttc ctgctcaccc
     2581 accctgtgct gagctttggt atccaggttg tgagtcgctg ccggctacgg cacactgagg
     2641 tgctgcctgc cgaagaggaa aatgacagcc tgggtgctga tggtacccat ggagccggtg
     2701 ccatggagtc tgcggccggt gtgctcatca agctcttttg tgtgcatact aaggcactgc
     2761 aagatgtgca gatccgcttc cagccacagc tgaaccctga tgtggtggcc ccactgccca
     2821 cccacactgc ccacgaggac ttcacatttg gagagtctcg gcccgaactg ggctctgagg
     2881 gcctggggtc agccgctcac ggctcccagc ctgacctccg acgaatcgtg gagctgcctg
     2941 cacctgccga cttcctcagt ctgagcagtg agaccaagcc caagttgatg acacctgacg
     3001 ccttcatgac acctagcgcc tccttgcagc agatcactgc ctctcccagc agcagcagca
     3061 gcggtagcag cagcagcagc agcagtagca gcagctccct tacagctgtg tctgccatga
     3121 gcagcacctc agctgtggac ccctccttga ccaggccacc tgaggagctg accttgagcc
     3181 ccaagctgca gctggatggc agcctgacaa tgagcagcag tggcagcctt caggcaagcc
     3241 cgcgtggcct cctgcctggc ctgctcccag ccccagctga caaactgact cccaaggggc
     3301 cgggccaggt gcctactgcc acctctgcac tgtccctgga gctgcaggaa gtggagcccc
     3361 tggggctacc ccaagcctcc cctagccgca ctcgttcccc tgatgtcatc tcctcagctt
     3421 ccactgccct gtcccaggac atccctgaga ttgcatctga ggccctgtcc cgtggttttg
     3481 gctcctctgc accagagggc cttgagccag acagtatggc ttcagccgcc tcggcactgc
     3541 acctgctgtc cccacggccc cggccagggc ccgagctcgg cccccagctc gggcttgatg
     3601 gaggccctgg ggatggagat cggcataata ccccctccct cctggaggca gccttgaccc
     3661 aggaggcctc gactcctgac agtcaggttt ggcccacagc acctgacatt actcgtgaga
     3721 cctgcagcac cctggcagaa agccccagga atggccttca ggaaaagcac aagagcctgg
     3781 ccttccaccg accaccatat cacctgctgc agcaacgtga cagccaggat gccagtgctg
     3841 agcaaagtga ccatgatgat gaggtggcca gccttgcctc tgcttcagga ggctttggca
     3901 ccaaagttcc tgctccacgg ctgcctgcca aggactggaa gaccaaggga tcccctcgaa
     3961 cctcacccaa gctcaagagg aaaagcaaga aggatgatgg ggatgcagcc atgggatccc
     4021 ggctcacaga gcaccaggtg gcagagcccc ctgaggactg gccagcacta atttggcaac
     4081 agcagagaga gctggcagag ctgcggcaca gccaggaaga gctgctgcag cgtctgtgta
     4141 cccaactcga aggcctgcag agcacagtca caggccacgt agaacgtgcc cttgagactc
     4201 ggcacgagca ggaacagcgg cggctggagc gagcactggc tgaggggcag cagcggggag
     4261 ggcagctgca ggagcagctg acacaacagt tgtcccaagc actgtcgtca gctgtagctg
     4321 ggcggctaga gcgcagcata cgggatgaga tcaagaagac agtccctcca tgtgtctcaa
     4381 ggagtctgga gcctatggca ggccaactga gcaactcagt ggctaccaag ctcacagctg
     4441 tggagggcag catgaaagag aacatctcca agctgctcaa gtccaagaac ttgactgatg
     4501 ccatcgcccg agcagctgca gacacattac aagggccgat gcaggctgcc taccgggaag
     4561 ccttccagag tgtggtgctg ccggcctttg agaagagctg ccaggccatg ttccagcaaa
     4621 tcaatgatag cttccggctg gggacacagg aatacttgca gcagctagaa agccacatga
     4681 agagccggaa ggcacgggaa caggaggcca gggagcctgt gctagcccag ctgcggggcc
     4741 tggtcagcac actgcagagt gccactgagc agatggcagc caccgtggcc ggcagtgttc
     4801 gtgctgaggt gcagcaccag ctgcatgtgg ctgtgggcag cctgcaggag tccattttag
     4861 cacaggtaca gcgcatcgtt aagggtgagg tgagtgtggc gctcaaggag cagcaggccg
     4921 ccgtcacctc cagcatcatg caggccatgc gctcagctgc tggcacacct gtcccctctg
     4981 cccaccttga ctgccaggcc cagcaagccc atatcctgca gctgctgcag cagggccacc
     5041 tcaatcaggc cttccagcag gcgctgacag ctgctgacct gaacctggtg ctgtatgtgt
     5101 gtgaaactgt ggacccagcc caggtttttg ggcagccacc ctgcccgctc tcccagcctg
     5161 tgctcctttc cctcatccag cagctggcat ctgaccttgg cactcgaact gacctcaagc
     5221 tcagctacct ggaagaggcc gtgatgcacc tggaccacag tgaccccatc actcgggacc
     5281 acatgggctc cgttatggcc caggtgcgcc aaaagctttt tcagttcctg caggctgagc
     5341 cacacaactc acttggcaaa gcagctcggc gtctcagcct catgctgcat ggcctcgtga
     5401 cccccagcct cccttaggcg gccgcttccc tttagtgagg gttaatgctt cgagcagaca
     5461 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5521 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5581 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     5641 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat ccgataagga tcgatccggg
     5701 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5761 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5821 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5881 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5941 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6001 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6061 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6121 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6181 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     6241 ttctccttac gcatctgtgc ggtatttcac accgcatacg cggatctgcg cagcaccatg
     6301 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca
     6361 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6421 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6541 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6601 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6661 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgatt cttctgacac
     6721 aacagtctcg aacttaaggc tagagccacc atgattgaac aagatggatt gcacgcaggt
     6781 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6841 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6901 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6961 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7021 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7081 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7141 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7201 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7261 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7321 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7381 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7441 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7501 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7561 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa taaaatatct
     7621 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatccgc
     7681 gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     7741 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7801 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7861 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7921 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7981 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8041 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8101 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8161 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8221 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8281 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8341 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8401 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8461 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8521 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8581 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8641 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8701 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8761 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8821 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8881 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8941 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9001 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9061 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9121 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9181 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9241 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9301 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9361 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9421 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9481 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9541 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9601 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9661 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9721 gccttttgct ggccttttgc tcacatggct cgacagatct
//
