LOCUS       sequence-11388-e       12485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 12485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-nucLacZ
FEATURES             Location/Qualifiers
     source          1..12485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3532..3552
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3565..6612
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(3567..3589)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     intron          7114..7179
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7309..7329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7601..7735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7638..7657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7692..7711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7762..7780)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8240..8311,8706..8979,9054..9708,9770..10085,
                     10306..10437,10508..11122)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(11900..12454,12455..12485)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgcggccgc ggctcgaggg tacctagacc accatgggag atcccacccc acccaagaag
     3541 aagcgcaagg tggaggacga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3601 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3661 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
     3721 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
     3781 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
     3841 accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
     3901 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
     3961 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
     4021 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
     4081 gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag
     4141 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
     4201 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
     4261 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
     4321 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
     4381 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
     4441 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
     4501 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
     4561 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
     4621 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
     4681 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
     4741 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
     4801 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
     4861 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
     4921 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
     4981 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
     5041 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
     5101 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
     5161 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
     5221 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
     5281 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
     5341 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
     5401 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
     5461 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
     5521 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
     5581 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
     5641 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
     5701 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
     5761 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
     5821 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
     5881 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
     5941 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
     6001 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
     6061 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
     6121 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
     6181 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
     6241 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
     6301 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
     6361 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
     6421 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
     6481 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
     6541 ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg
     6601 tgtcaaaaat aataataacc gggcaggggg gatcgatccc ccagatccgt acgatcctct
     6661 agtaaaaaaa aaacaaaatt ttagtgtgta taattaggct aaacaactgg aggattcatt
     6721 gctccctttg agtttatact ttttgtaaca agaacaggcg caacactcgg gagaattttg
     6781 catttcattt gtgaataaac gaatacaata aatacaaata cacgaaaaga agtcagctgt
     6841 cgagtttttg ttggtttttt tttccctttt tagcttcgaa tctgcaaatg tatttgcatt
     6901 taagtaaacc ggcttttgtt gggtgtcgtt aactttcaag gggtcttatg tacttattct
     6961 gttttccttg tccttgaatt ggacttgtgg aaaattaaat ggtcagccaa ttgcttaacc
     7021 tttgtcgagc tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     7081 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     7141 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     7201 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     7261 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     7321 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     7381 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     7441 ctgctataca agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat
     7501 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7561 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     7621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7681 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcggatc
     7741 cactagaagg ccttagtatg tatgtaagtt aataaaaccc ttttttggag aatgtagatt
     7801 taaaaaaaca tatttttttt ttatttttta ctgcactgga catcattgaa cttatctgat
     7861 cagttttaaa tttacttcga tccaagggta tttgaagtac caggttcttt cgattacctc
     7921 tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga
     7981 aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt
     8041 acctagcgtc aatgtccgcc ttcagttgca ctttgtcagc ggtttcgtga cgaagctcca
     8101 agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat
     8161 ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa
     8221 agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag
     8281 caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag
     8341 ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc
     8401 gacccacaaa aatcccaaac cgcaatcgca caaacaaata gtgacacgaa acagattatt
     8461 ctggtagctg tgctcgctat ataagacaat ttttaagatc atatcatgat caagacatct
     8521 aaaggcattc attttcgact acattctttt ttacaaaaaa tataacaacc agatatttta
     8581 agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc
     8641 gttttgttcg gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat
     8701 tgcagggtga cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca
     8761 aaaactacgg cacgctcctg ccacccagtc cgccggagga ctccggttca gggagcggcc
     8821 aactagccga gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc
     8881 agccgggctc cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc
     8941 gacacatacc ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg
     9001 gctgatctgt gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg
     9061 cgtggcctat ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac
     9121 cctgctgaat gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat
     9181 gcgactgctc aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt
     9241 ccagcaggat gacctcttta tcggctccct aacggccagg gaacacctga ttttccaggc
     9301 catggtgcgg atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt
     9361 gatccaggag ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt
     9421 gaaaggtctg tccggcggag aaaggaagcg tctggcattc gcctccgagg cactaaccga
     9481 tccgccgctt ctgatctgcg atgagcccac ctccggactg gactcattta ccgcccacag
     9541 cgtcgtccag gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca
     9601 tcagccgtct tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag
     9661 ggtagctttc ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg
     9721 tgtttattaa gggtatctag cattacatta catctcaact cctatccagc gtgggtgccc
     9781 agtgtcctac caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg
     9841 gacgggagat cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gctattagca
     9901 aagtagcccg ggatatggag cagttgttgg ccaccaaaaa tttggagaag ccactggagc
     9961 agccggagaa tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt
    10021 ggcgatcctg gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga
    10081 caacggtgag tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa
    10141 ttcgctagat tttagttaga attgcctgat tccacaccct tcttagtttt tttcaatgag
    10201 atgtatagtt tatagttttg cagaaaataa ataaatttca tttaactcgc gaacatgttg
    10261 aagatatgaa tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg
    10321 attggcctca tctttttggg ccaacaactc acgcaagtgg gcgtgatgaa tatcaacgga
    10381 gccatcttcc tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta
    10441 agtcttgttt agaatacatt tgcatattaa taatttacta actttctaat gaatcgattc
    10501 gatttaggtg ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta
    10561 tcgctgtgac acatactttc tgggcaaaac gattgccgaa ttaccgcttt ttctcacagt
    10621 gccactggtc ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca
    10681 cttcttcaac tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata
    10741 tctaatatcc tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat
    10801 cataccattc ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct
    10861 caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca
    10921 atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag
    10981 ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta
    11041 cgtgggtctg gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact
    11101 tcgggcccga cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt
    11161 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
    11221 ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc
    11281 ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac
    11341 atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata tacatacttt
    11401 tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt
    11461 tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg
    11521 actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt tcatttcgtc
    11581 tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa tatatttgta
    11641 tatatttccc tcacagctat atttattcta atttaatatt atgacttttt aaggtaattt
    11701 tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac atccagtgtt
    11761 tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg tttatatata
    11821 tcaaaaataa caactataat aataagaata catttaattt agaaaatgct tggatttcac
    11881 tggaactaga attaattcgg ctgctgctct aaacgacgca tttcgtactc caaagtacga
    11941 attttttccc tcaagctctt attttcatta aacaatgaac aggacctaac gcacagtcac
    12001 gttattgttt acataaatga ttttttttac tattcaaact tactctgttt gtgtactccc
    12061 actggtatag ccttctttta tcttttctgg ttcaggctct atcactttac taggtacggc
    12121 atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag ccttccactg
    12181 cgaatcatta aagtgggtat cacaaatttg ggagttttca ccaaggctgc acccaaggct
    12241 ctgctcccac aattttctct taatagcaca cttcggcacg tgaattaatt ttactccagt
    12301 cacagctttg cagcaaaatt tgcaatattt catttttttt tattccacgt aagggttaat
    12361 gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct caacaagcaa acgtgcactg
    12421 aatttaagtg tatacttcgg taagcttcgg ctatcgacgg gaccacctta tgttatttca
    12481 tcatg
//
