LOCUS       sequence-113886-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FokI-dCas9 and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T
  TITLE     Production of knockout mice by DNA microinjection of various 
            CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
  JOURNAL   BMC Biotechnol. 2015 May 22;15(1):33.
  PUBMED    25997509
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-05-22"; volume: "15(1)"; pages: "33"
COMMENT     Sequence Label: pX330A_FokI-1x4
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(302..889)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag cagatccggt
       61 tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc tcggctgttt
      121 cgccgctgtc gaacagcagg gctccgatca ggttcttctt gatgctgtgc cggtcggtgt
      181 tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc acggcccagc
      241 ccacagagtt ggtgccgatg gccaggccga tgctgtactt cttgtcggat ccaccgccac
      301 cgaaattgat ctcgccattg ttaaacttgc gtctgacttc ttccagtgtc agagttcccg
      361 ctttgatcat ttctccacca atgagcagtt cctctacgga caaaaccgca ccattgcagt
      421 tcgtaatgtg attcaaccgt gtgagctggg ccttatagtt gcctttgaaa tgcccagaga
      481 caaacaaaaa cttaaactcg gtcactgatg aaggatacac tttccaccac tcatttggat
      541 tgatgtgttt gttcctggtc tgattttctt cgacataacg ttgcatctca tctgcctgcc
      601 cgatgggaag attgtaccca ccgctgtagg ctttcgtgtc gacgatcact ccgtaatcaa
      661 tcgggctccc caccgtatag attgctccgt cgggttttcg tgatccaccg agatgcttcc
      721 ctcggtatcc ataaaccttc ataaagaact ccattacctt catctcaagg attctgtctt
      781 gagtcgagtt cctagcaatt tcgataagtt cgatgtattc atgcggtacg tatttcaatt
      841 tgtgccgcag ctccgacttt ttctcctcaa gttcactctt cacgagttgg gagctcacct
      901 tccgcttc
//
