LOCUS       sequence-113889-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5′ TOP TRICK reporter mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pSF4 CMV 5TOP intron renilla TRICK CTE polyA
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..41
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    149..283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(186..205)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctattccgaa gttcctattc ttcaaatagt ataggaactt cagatcataa tcagccatac
       61 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      121 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      181 ataaggcaat agcatcacaa atttcacaaa taaggcattt ttttcactgc attctagttt
      241 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctcaaatc cctcggaagc
      301 tgcgcctgtc ttaggttgga gtgatacatt tttatcactt ttacccgtct ttggattagg
      361 cagtagctct gacggccctc ctgtcttagg ttagtgaaaa atgtcactct cttacccgtc
      421 attggctgtc cagcttagct cgcaggggag gtggtctgga tcccgcgcga aatcgattga
      481 agatctggta tgtccgatgt tgcgacggct gatgctcgtc gcagaagagt cgataacgta
      541 gaggcagtag gagcggctgc cgtgatcgtg cagccgtttg atgatagtgt ctgaggcatg
      601 ctgtggacgc tcgcgtgatc gtcgagcgtt gaatgatgga tgtcctgatg tagtcggagc
      661 tccagcgctg atgctgctgg agtttgattg gataagactg taatgacagt ggagcgtgcc
      721 gtgatcgtgc acgcttgaag attggacagt gatgaagatg ctgggcggca cggctgatgc
      781 tcgtgccgtt tgtaggtagg atctggtatg tccgatgttg cgacggctga tgctcgtcgc
      841 agaagagtcg ataacgtaga ggcagtagga gcggctgccg tgatcgtgca gccgtttgat
      901 gatagtgtct gaggcatgct gtggacgctc gcgtgatcgt cgagcgttga atgatggatg
      961 tcctgatgt
//
