LOCUS       sequence-113894-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector for lncRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Alvarez-Dominguez JR, Bai Z, Xu D, Yuan B, Lo KA, Yoon MJ, Lim YC, 
            Knoll M, Slavov N, Chen S, Chen P, Lodish HF, Sun L
  TITLE     De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long
            Non-coding RNA Regulators of Brown Adipocyte Development.
  JOURNAL   Cell Metab. 2015 May 5;21(5):764-76. doi: 
            10.1016/j.cmet.2015.04.003. Epub 2015 Apr 23.
  PUBMED    25921091
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.04.003"; journalName: "Cell Metab"; date: 
            "2015-05-5- 5"; volume: "21"; issue: "5"; pages: "764-76"
COMMENT     Sequence Label: pLncEXP
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(210..413)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(239..259)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        414..717
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(812..828)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(844..866)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ctagcagcag ggcgcctcca ccacgcacag gttgatggcc tgcttgccct tgaaggggta
       61 gccgatgccc tcgccggtga tcacgaactt gtggccgtcc acgcagccct ccatgcggta
      121 cttcatggtc atctccttgg tcaggccgtg cttggactgg gccatggtgg cgaccggtag
      181 cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
      241 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
      301 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      361 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
      421 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      481 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
      541 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
      601 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
      661 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
      721 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagaa
      781 ttgatcttca attccggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg
      841 cggggtcgaa cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg
      901 aggcttaggg tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca
      961 ggtcacgatg taagggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat
     1021 cttaacctgg gtgatgaagt ctcggttaaa ggtgccgtct cccggccatc ccaacgttaa
     1081 aggttggcca ttctgcaaag cagaaggtaa cccaacgtct ctttttgaat ctaccgactg
     1141 gg
//
