LOCUS       sequence-113897-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a 
            T7-RFP tag, invariable V5 tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, 
            Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F
  TITLE     Recombination-induced tag exchange to track old and new proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 
            10.1073/pnas.0911164107. Epub 2009 Dec 14.
  PUBMED    20018668
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0911164107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-5- 5"; volume: "107"; issue: "1"; pages: "64-8"
COMMENT     Sequence Label: pKV015
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..115
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    122..155
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             164..190
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             197..910
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             197..910
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cgactcacta tagggcgaat tgggcccaat gcattggcgc cgcggccgcg gtggatctgg
       61 tggatctgct agcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgagcgc
      121 tataacttcg tataatgtat gctatacgaa gttatcaggt acctacccat acgatgttcc
      181 tgactatgcg actagtatgt ctaaaggtga agaattattc actggtgttg tcccaatttt
      241 ggttgaatta gatggtgatg ttaatggtca caaattttct gtctccggtg aaggtgaagg
      301 tgatgctact tacggtaaat tgaccttaaa atttatttgt actactggta aattgccagt
      361 tccatggcca accttagtca ctactttaac ttatggtgtt caatgttttt ctagataccc
      421 agatcatatg aaacaacatg actttttcaa gtctgccatg ccagaaggtt atgttcaaga
      481 aagaactatt tttttcaaag atgacggtaa ctacaagacc agagctgaag tcaagtttga
      541 aggtgatacc ttagttaata gaatcgaatt aaaaggtatt gattttaaag aagatggtaa
      601 cattttaggt cacaaattgg aatacaacta taactctcac aatgtttaca tcatggctga
      661 caaacaaaag aatggtatca aagttaactt caaaattaga cacaacattg aagatggttc
      721 tgttcaatta gctgaccatt atcaacaaaa tactccaatt ggtgatggtc cagtcttgtt
      781 accagacaac cattacttat ccactcaatc tgccttatcc aaagatccaa acgaaaagag
      841 agaccacatg gtcttgttag aatttgttac tgctgctggt attacccatg gtatggatga
      901 attgtataaa actagttaac ttctaaataa gcgaattc
//
