LOCUS       sequence-113899-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Zeo-ecfp
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     243..261
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    307..323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    340..356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             375..386
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(438..459)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc
       61 acttatctgg ttggcctgca aggccttcgc gaggtaccat ttatcagggt tattgtctca
      121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      181 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      241 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gaaaatttat caaaaagagt
      301 gttgacttgt gagcggataa caatgatact tagattcaat tgtgagcgga taacaatttc
      361 acacatctag aattaaagag gagaaattaa gcatgctgag caagggcgag gagctgttca
      421 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      481 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      541 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc
      601 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      661 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tac
//
