LOCUS       sequence-11390-e       10053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAS-DSCP-FLPo
FEATURES             Location/Qualifiers
     source          1..10053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     intron          4682..4747
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4877..4897
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5169..5303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5206..5225)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5260..5279
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5330..5348)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5808..5879,6274..6547,6622..7276,7338..7653,7874..8005,
                     8076..8690)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9468..10022,10023..10053)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattgga gctcgcccgg ggatcgagcg
     3181 cagcggtata aaagggcgcg gggtggctga gagcatcagt tgtgaatgaa tgttcgagcc
     3241 gagcagacgt gccgctgcct tcgttaatat cctttgaata agccaacttt gaatcacaag
     3301 acgcatacca aacgaattcc aacatgagcc agttcgacat cctgtgcaag acccccccca
     3361 aggtgctggt gcggcagttc gtggagagat tcgagaggcc cagcggcgag aagatcgcca
     3421 gctgtgccgc cgagctgacc tacctgtgct ggatgatcac ccacaacggc accgccatca
     3481 agagggccac cttcatgagc tacaacacca tcatcagcaa cagcctgagc ttcgacatcg
     3541 tgaacaagag cctgcagttc aagtacaaga cccagaaggc caccatcctg gaggccagcc
     3601 tgaagaagct gatccccgcc tgggagttca ccatcatccc ttacaacggc cagaagcacc
     3661 agagcgacat caccgacatc gtgtccagcc tgcagctgca gttcgagagc agcgaggagg
     3721 ccgacaaggg caacagccac agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga
     3781 gcatctggga gatcaccgag aagatcctga acagcttcga gtacaccagc aggttcacca
     3841 agaccaagac cctgtaccag ttcctgttcc tggccacatt catcaactgc ggcaggttca
     3901 gcgacatcaa gaacgtggac cccaagagct tcaagctggt gcagaacaag tacctgggcg
     3961 tgatcattca gtgcctggtg accgagacca agacaagcgt gtccaggcac atctactttt
     4021 tcagcgccag aggcaggatc gaccccctgg tgtacctgga cgagttcctg aggaacagcg
     4081 agcccgtgct gaagagagtg aacaggaccg gcaacagcag cagcaacaag caggagtacc
     4141 agctgctgaa ggacaacctg gtgcgcagct acaacaaggc cctgaagaag aacgccccct
     4201 accccatctt cgctatcaag aacggcccta agagccacat cggcaggcac ctgatgacca
     4261 gctttctgag catgaagggc ctgaccgagc tgacaaacgt ggtgggcaac tggagcgaca
     4321 agagggcctc cgccgtggcc aggaccacct acacccacca gatcaccgcc atccccgacc
     4381 actacttcgc cctggtgtcc aggtactacg cctacgaccc catcagcaag gagatgatcg
     4441 ccctgaagga cgagaccaac cccatcgagg agtggcagca catcgagcag ctgaagggca
     4501 gcgccgaggg cagcatcaga taccccgcct ggaacggcat catcagccag gaggtgctgg
     4561 actacctgag cagctacatc aacaggcgga tctgataatc tagaggatct ttgtgaagga
     4621 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     4681 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     4741 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     4801 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     4861 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     4921 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     4981 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt
     5041 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     5101 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5161 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5221 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5281 atgtatctta tcatgtctgg atcggatcca ctagaaggcc ttagtatgta tgtaagttaa
     5341 taaaaccctt ttttggagaa tgtagattta aaaaaacata tttttttttt attttttact
     5401 gcactggata tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt
     5461 tgaagtacca ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc
     5521 gctgtttgcc tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc
     5581 tatctctttc gccaccgttt gtagcgttac ctagcgtcaa tgtccgcctt cagttgcact
     5641 ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg
     5701 ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg
     5761 attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg
     5821 atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg
     5881 tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac
     5941 atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caatcgcaca
     6001 aacaaatagt gacacgaaac agattattct ggtagctgtg ctcgctatat aagacaattt
     6061 ttaagatcat atcatgatca agacatctaa aggcattcat tttcgactac attctttttt
     6121 acaaaaaata taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa
     6181 agtataaacc tacttcgtag gatacttcgt tttgttcggg gttagatgag cataacgctt
     6241 gtagttgata tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc
     6301 tgcattaacc agggcttcgg gcaggccaaa aactacggca cgctcctgcc acccagtccg
     6361 ccggaggact ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac
     6421 aatatggaca tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac
     6481 cggacacgcg gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc
     6541 aagaacggtg agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc
     6601 aattaccaat ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga
     6661 tgggcagttc cggtgccgga aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc
     6721 agggcatcca agtatcgcca tccgggatgc gactgctcaa tggccaacct gtggacgcca
     6781 aggagatgca ggccaggtgc gcctatgtcc agcaggatga cctctttatc ggctccctaa
     6841 cggccaggga acacctgatt ttccaggcca tggtgcggat gccacgacat ctgacctatc
     6901 ggcagcgagt ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc aaatgtcagc
     6961 acacgatcat cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc
     7021 tggcattcgc ctccgaggca ctaaccgatc cgccgcttct gatctgcgat gagcccacct
     7081 ccggactgga ctcatttacc gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga
     7141 agggcaagac cgtcatcctg accattcatc agccgtcttc cgagctgttt gagctctttg
     7201 acaagatcct tctgatggcc gagggcaggg tagctttctt gggcactccc agcgaagccg
     7261 tcgacttctt ttcctagtga gttcgatgtg tttattaagg gtatctagca ttacattaca
     7321 tctcaactcc tatccagcgt gggtgcccag tgtcctacca actacaatcc ggcggacttt
     7381 tacgtacagg tgttggccgt tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc
     7441 aagatatgcg acaattttgc tattagcaaa gtagcccggg atatggagca gttgttggcc
     7501 accaaaaatt tggagaagcc actggagcag ccggagaatg ggtacaccta caaggccacc
     7561 tggttcatgc agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca
     7621 ctcctcgtaa aagtgcgact tattcagaca acggtgagtg gttccagtgg aaacaaatga
     7681 tataacgctt acaattcttg gaaacaaatt cgctagattt tagttagaat tgcctgattc
     7741 cacacccttc ttagtttttt tcaatgagat gtatagttta tagttttgca gaaaataaat
     7801 aaatttcatt taactcgcga acatgttgaa gatatgaata ttaatgagat gcgagtaaca
     7861 ttttaatttg cagatggttg ccatcttgat tggcctcatc tttttgggcc aacaactcac
     7921 gcaagtgggc gtgatgaata tcaacggagc catcttcctc ttcctgacca acatgacctt
     7981 tcaaaacgtc tttgccacga taaatgtaag tcttgtttag aatacatttg catattaata
     8041 atttactaac tttctaatga atcgattcga tttaggtgtt cacctcagag ctgccagttt
     8101 ttatgaggga ggcccgaagt cgactttatc gctgtgacac atactttctg ggcaaaacga
     8161 ttgccgaatt accgcttttt ctcacagtgc cactggtctt cacggcgatt gcctatccga
     8221 tgatcggact gcgggccgga gtgctgcact tcttcaactg cctggcgctg gtcactctgg
     8281 tggccaatgt gtcaacgtcc ttcggatatc taatatcctg cgccagctcc tcgacctcga
     8341 tggcgctgtc tgtgggtccg ccggttatca taccattcct gctctttggc ggcttcttct
     8401 tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt
     8461 acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct
     8521 gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact
     8581 tctccgccgc cgatctgccg ctggactacg tgggtctggc cattctcatc gtgagcttcc
     8641 gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag ccgacatata
     8701 tccgaaataa ctgcttgttt ttttttttac cattattacc atcgtgttta ctgtttattg
     8761 ccccctcaaa aagctaatgt aattatattt gtgccaataa aaacaagata tgacctatag
     8821 aatacaagta tttccccttc gaacatcccc acaagtagac tttggatttg tcttctaacc
     8881 aaaagactta cacacctgca taccttacat caaaaactcg tttatcgcta cataaaacac
     8941 cgggatatat tttttatata catacttttc aaatcgcgcg ccctcttcat aattcacctc
     9001 caccacacca cgtttcgtag ttgctctttc gctgtctccc acccgctctc cgcaacacat
     9061 tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct
     9121 caaatggttc cgagtggttc atttcgtctc aatagaaatt agtaataaat atttgtatgt
     9181 acaatttatt tgctccaata tatttgtata tatttccctc acagctatat ttattctaat
     9241 ttaatattat gactttttaa ggtaattttt tgtgacctgt tcggagtgat tagcgttaca
     9301 atttgaactg aaagtgacat ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg
     9361 gtgggcataa tagtgttgtt tatatatatc aaaaataaca actataataa taagaataca
     9421 tttaatttag aaaatgcttg gatttcactg gaactagaat taattcggct gctgctctaa
     9481 acgacgcatt tcgtactcca aagtacgaat tttttccctc aagctcttat tttcattaaa
     9541 caatgaacag gacctaacgc acagtcacgt tattgtttac ataaatgatt ttttttacta
     9601 ttcaaactta ctctgtttgt gtactcccac tggtatagcc ttcttttatc ttttctggtt
     9661 caggctctat cactttacta ggtacggcat ctgcgttgag tcgcctcctt ttaaatgtct
     9721 gaccttttgc aggtgcagcc ttccactgcg aatcattaaa gtgggtatca caaatttggg
     9781 agttttcacc aaggctgcac ccaaggctct gctcccacaa ttttctctta atagcacact
     9841 tcggcacgtg aattaatttt actccagtca cagctttgca gcaaaatttg caatatttca
     9901 ttttttttta ttccacgtaa gggttaatgt tttcaaaaaa aaattcgtcc gcacacaacc
     9961 tttcctctca acaagcaaac gtgcactgaa tttaagtgta tacttcggta agcttcggct
    10021 atcgacggga ccaccttatg ttatttcatc atg
//
