LOCUS       sequence-113901-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Zeo-gfpmut3
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             239..250
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(287..315)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
       61 atttcactta tctggttggc ctgcaaggcc ttcgcgaggt accgggccca agcttctcga
      121 ggaattcctg cagcccgggg gatccactag tgagctcgtt gcgcggtcag aaaattattt
      181 taaatttcct ctagtcaggc cggaataact ccctataatg cgacaccact ctagaattaa
      241 agaggagaaa ttaagcatgc gtaaaggaga agaacttttc actggagttg tcccaattct
      301 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      361 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      421 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      481 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      541 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      601 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      661 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      721 c
//
