LOCUS       sequence-113902-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Zeo-dsRedExpress
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    114..130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    147..163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             182..193
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(380..400)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc
       61 acttatctgg ttggcctgca aggtcgagaa aatttatcaa aaagagtgtt gacttgtgag
      121 cggataacaa tgatacttag attcaattgt gagcggataa caatttcaca catctagaat
      181 taaagaggag aaattaagca tggcctcctc cgagaacgtc atcaccgagt tcatgcgctt
      241 caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      301 gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgaccaagg gcggccccct
      361 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa
      421 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga
      481 gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg acccaggact cctccctgca
      541 ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc
      601 cgtgatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt a
//
