LOCUS       sequence-113904-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 
            epitope tag, invariable V5 tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pKV006
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(62..94)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(103..136)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        231..574
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 agctcgaaat taaccctcac taaagggaac aaaagcttgc atgcgcggcc gcttatgtac
       61 aacccatttg ctgtccacca gtcatgcttg ccatgtcgac tgataacttc gtatagcata
      121 cattatacga agttattcta gactgtgcgg tatttcacac cgcatagggt taattaaggc
      181 gcgccagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
      241 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
      301 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
      361 gccgcacggc gcgaagcaaa aattacggct cctcgctgcg gacctgcgag cagggaaacg
      421 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
      481 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
      541 acagttctca catcacatcc gaacataaac aaccatgggt aaaaagcctg aactcaccgc
      601 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
      661 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
      721 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
      781 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
      841 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
      901 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
      961 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1021 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1081 caccgtcagt gcgtccgtcg cgcaggct
//
