LOCUS       sequence-113906-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV116.loxP
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    202..235
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
ORIGIN
        1 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
       61 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      121 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcggtctc tactgggggc
      181 tgcaggaatt cgccacgtcc cataacttcg tataatgtac attatacgaa gttatcccag
      241 ctgccgtcga cgctcgagga ggggtaataa ctgatataat taaattgaag ctctaatttg
      301 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
      361 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
      421 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag acaccacatc
      481 atccacggtt ctatactgtt gacccaatgc atctcccttg tcatctaaac ccacaccggg
      541 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
      601 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
      661 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
      721 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
      781 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
      841 gactgtatta ccaatgtcag caaattt
//
