LOCUS       sequence-113907-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For integration of Cre recombinase at the yeast URA3 locus orthe 
            CYC1 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pSS146
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..1094
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEAAHRLIYGAEDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVVLLE
                     DGD"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcgt
       61 ccggaatgtc caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa
      121 cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg
      181 agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga
      241 ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc
      301 aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc
      361 atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc
      421 ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac
      481 gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac
      541 gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca
      601 ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca
      661 gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta
      721 aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt
      781 tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc
      841 gcgccctgga agggattttt gaagcagctc atcgactgat ttacggcgct gaggatgact
      901 ctggtcagag gtacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata
      961 tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa
     1021 atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtggtcctgc
     1081 tggaagatgg cgatc
//
