LOCUS       sequence-113908-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GmR mini-Tn7 vector, R6K replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18R6K-mini-Tn7T-Gm
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      121..477
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
       61 gttatccaca gaatcagggg ataacgcagg aaagaacatg ggcagttcaa cctgttgata
      121 gtacgtacta agctctcatg tttcacgtac taagctctca agtttaacgt actaagctct
      181 catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta
      241 agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag
      301 caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt
      361 ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa
      421 gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg gctgacaacc
      481 ggctggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      541 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      661 cggtggtttt tttgtttgca agcagcagat tacgcgca
//
