LOCUS       sequence-113911-         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For fungal transformation via ATMT. Transformants to be selected by 
            bialaphos resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Sweigard JA, Chumley F, Carroll A, Farrall L, Valent B
  TITLE     A series of vectors for fungal transformation
  JOURNAL   Fungal Genetics Reports, 1997. Vol. 44, Article 19
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genetics Reports, 1997. Vol. 44, Article 19"
COMMENT     Sequence Label: pFGL822
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..547)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTE"
ORIGIN
        1 tctcggtgac gggcaggacc ggacggggcg gaaccggcag gctgaagtcc agctgccaga
       61 aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg
      121 catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga
      181 ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc
      241 ccagtcccgt ccgctggtgg cggggggaga cgtacacggt cgattcggcc gtccagtcgt
      301 aggcgttgcg tgccttccag ggacccgcgt aggcgatgcc ggcgacctcg ccgtccacct
      361 cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg
      421 gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg
      481 tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg
      541 ggctcatggt tacttcctaa tcgaagcgtt gcttgggtag
//
