LOCUS       sequence-113914-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an 
            HA-6xHIS epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pMT006
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..93
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             102..149
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     terminator      170..357
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(225..249)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(476..673)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tatagggcga attgggccca atgcattggc gccgcggccg cggtggatct ggtggatcta
       61 taacttcgta taatgtatgc tatacgaagt tatcaggtac cgactacaag gacgatgacg
      121 ataaagatta taaagatgac gatgacaaga ctagttaact tctaaataag cgaatttctt
      181 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
      241 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
      301 caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct ctaccggcag
      361 atccgctagg gataacaggg taaggatcca gattgtactg agagtgcacc ataatcatcg
      421 atgaattcga gctcgttttc gacactggat ggcggcgtta gtatcgaatc gacagcagta
      481 tagcgaccag cattcacata cgattgacgc atgatattac tttctgcgca cttaacttcg
      541 catctgggca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca tttgattaaa
      601 atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa acaagaatct
      661 ttttattgtc agtactgatt attcctttgc cctcggacga gtgctggggc gtcggtttcc
      721 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
      781 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
      841 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
      901 gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
      961 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     1021 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgc
//
