LOCUS       sequence-113917-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway vector for generating green fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Gg
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..162
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(203..508)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(305..324)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatca ggatcccggg ccctatcacc actttgtaca agaaagctga
       61 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      121 gactacataa tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc
      181 tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga
      241 tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac
      301 tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa
      361 gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc
      421 gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc
      481 ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag
      541 ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag
      601 cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata
      661 ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac
      721 gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag tatatattct
      781 tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga
      841 tccacgcgtt tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      901 tctgccgaca tgg
//
