LOCUS       sequence-113928-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 
            epitope tag, invariable Myc tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pKV005
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..91
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    98..131
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             140..166
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      187..374
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(242..266)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(493..690)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gggcgaattg ggcccaatgc attggcgccg cggccgcggt ggatctggtg gatctgctag
       61 cgaacaaaag ttgatttctg aagaagattt gagcgctata acttcgtata atgtatgcta
      121 tacgaagtta tcaggtacct acccatacga tgttcctgac tatgcgacta gttaacttct
      181 aaataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
      241 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt
      301 aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac
      361 cacacctcta ccggcagatc cgctagggat aacagggtaa ggatccagat tgtactgaga
      421 gtgcaccata atcatcgatg aattcgagct cgttttcgac actggatggc ggcgttagta
      481 tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg atattacttt
      541 ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac
      601 gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa atgacaagtt
      661 cttgaaaaca agaatctttt tattgtcagt actgattatt cctttgccct cggacgagtg
      721 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
      781 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
      841 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
      901 agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc
      961 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     1021 aagaagatgt tggcgacctc gtattgggaa tccccgaa
//
