LOCUS       sequence-11393-e       11651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 11651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-shibire (ts1)
FEATURES             Location/Qualifiers
     source          1..11651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     3491..3507
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3491..3507
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      3517..3526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4231..4242
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6172..6188)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6173..6189)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          6280..6345
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6475..6495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6767..6901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6804..6823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6858..6877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6928..6946)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7406..7477,7872..8145,8220..8874,8936..9251,9472..9603,
                     9674..10288)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(11066..11620,11621..11651)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgcggccgc tctagaacta gtggatccaa ggagccgccg ccatggatag tttaattaca
     3541 attgttaaca agctgcaaga tgcgttcaca tcgctcggcg tccacatgca actggatctg
     3601 ccgcagatcg cggtggtcgg tggccagtca gctggcaaga gttccgtttt ggagaacttt
     3661 gtgggcaaag atttcttgcc gcgcggctct ggtatcgtaa ctcggagacc actaatcttg
     3721 caactgatca atggagttac cgaatatggc gagttccttc acattaaagg caagaagttt
     3781 tccagcttcg atgagatcag aaaagaaatc gaagatgaaa cggatcgtgt gaccggtagt
     3841 aacaaaggca tttcaaacat tccaattaat ttgcgcgtct actcgccgca cgtactcaat
     3901 ttaaccctga tcgatttgcc cggcctaaca aaggtggcca ttggcgatca accggtggat
     3961 attgagcagc aaattaagca gatgatattc caatttattc gcaaagagac ttgtttaatt
     4021 ttggctgtga ccccggccaa tacggatctg gccaattcgg atgccctcaa gctggccaag
     4081 gaggtggatc cacagggtgt gcgaaccatt ggcgttataa ccaagctgga tctgatggac
     4141 gagggcaccg atgcacgcga cattctggag aataagctgc ttccgctgcg acgtggctat
     4201 attggcgtgg tgaatagatc ccaaaaggat atcgaggggc gcaaggatat ccaccaggca
     4261 ttggccgccg aacgcaagtt cttcttgagc catccctcct atcggcacat ggccgaccgt
     4321 ctcgacaccc cctacttgca gcgcgtgctc aaccaacaac taaccaatca catcagggac
     4381 acgttacctg gcctgcggga taagttgcag aagcagatgc tcactttgga gaaggaggtg
     4441 gaggagttta agcactttca gccaggcgat gctagcatca aaacaaaagc tatgctccaa
     4501 atgattcagc agctgcaatc agactttgag cgaactatcg agggcagcgg ctcagctttg
     4561 gtgaacacaa acgagctctc aggtggcgcc aagatcaatc gcattttcca tgaacgtctg
     4621 cgctttgaga tcgtaaagat ggcttgcgat gaaaaggaac tgcgacgaga gatctcattt
     4681 gcaattcgaa atatccatgg tattcgcgtt ggcctcttca cacccgacat ggcgttcgag
     4741 gcgattgtca agcggcagat agcgctgctc aaggagccgg ttatcaagtg tgtcgatcta
     4801 gtcgtacagg aactttctgt ggtcgtgcgc atgtgcacag ctaaaatgag tcgataccca
     4861 cgtctgcgtg aggagaccga acgtattatc acaacacatg tgcgccaacg cgagcatagc
     4921 tgcaaggaac agatcctgct gcttattgac tttgagctgg cctatatgaa caccaatcat
     4981 gaggacttca ttggcttcgc caacgctcaa aacaaatcag agaatgccaa caaaactggc
     5041 actcgtcaac tgggcaacca agtcattcgc aagggtcaca tggtcatcca gaaccttgga
     5101 atcatgaaag gtggatcgcg tccgtattgg tttgtgctga catccgagag tatctcctgg
     5161 tataaggacg aggatgagaa ggagaagaaa tttatgctgc cgttggatgg tttaaaattg
     5221 cgtgatattg aacagggatt tatgtcaatg tctagacgtg ttacatttgc tttatttagt
     5281 cccgatggac gtaatgttta taaggactac aaacaactgg agctctcgtg cgagactgtg
     5341 gaggatgtgg agtcctggaa agcatccttc ctgcgagctg gtgtctatcc cgaaaagcag
     5401 gagactcagg aaaacggcga tgaggaagga caagagcaaa aatcggccag cgaggagagt
     5461 tccagcgatc cacagctgga gcggcaggtc gagaccattc gtaatctggt tgattcctac
     5521 atgaagatcg ttacgaagac cacccgtgac atggtgccca aagcaattat gatgctgatc
     5581 atcaacaatg ccaaggactt tatcaatggc gagctgctgg cacatcttta cgcgtccggc
     5641 gatcaggccc agatgatgga ggagtcggct gaatcggcaa cacggcgaga ggaaatgctg
     5701 cgcatgtacc gtgcttgcaa ggatgcatta cagataatag gtgacgtatc aatggccacc
     5761 gtgtcgtcgc cattgccacc gccggttaag aacgattggc tgcccagcgg cttggacaac
     5821 ccacgtcttt ctcctccaag tccgggtggc gttcgaggaa agccaggacc tcccgcacag
     5881 agctccttgg gtggacggaa cccaccattg ccaccatcta caggtcgccc agcgccagcg
     5941 attccgaatc gcccaggagg tggggcacca ccactacccg gcggtcgtcc cggaggatct
     6001 cttcccccac caatgctacc atcgcgtcgt taaattgttg tcagcgtcga gagacatcta
     6061 catatgtatc taggggatga atctgcaaaa cgtagcttgc tgacgattta accaaaccac
     6121 aaacaatacc caaattctac tttcaacaat tgtctaagtt aacaagctta tcgataccgt
     6181 cgacctcgag ggtacctcta gaggatcttt gtgaaggaac cttacttctg tggtgtgaca
     6241 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     6301 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     6361 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     6421 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     6481 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     6541 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     6601 gctgcactgc tatacaagaa aattatggaa aaatatttga tgtatagtgc cttgactaga
     6661 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     6721 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     6781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     6901 cggatccact agaaggcctt agtatgtatg taagttaata aaaccctttt ttggagaatg
     6961 tagatttaaa aaaacatatt ttttttttat tttttactgc actggacatc attgaactta
     7021 tctgatcagt tttaaattta cttcgatcca agggtatttg aagtaccagg ttctttcgat
     7081 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     7141 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     7201 agcgttacct agcgtcaatg tccgccttca gttgcacttt gtcagcggtt tcgtgacgaa
     7261 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     7321 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     7381 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     7441 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     7501 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     7561 aactgcgacc cacaaaaatc ccaaaccgca atcgcacaaa caaatagtga cacgaaacag
     7621 attattctgg tagctgtgct cgctatataa gacaattttt aagatcatat catgatcaag
     7681 acatctaaag gcattcattt tcgactacat tcttttttac aaaaaatata acaaccagat
     7741 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     7801 tacttcgttt tgttcggggt tagatgagca taacgcttgt agttgatatt tgagatcccc
     7861 tatcattgca gggtgacagc ggagcggctt cgcagagctg cattaaccag ggcttcgggc
     7921 aggccaaaaa ctacggcacg ctcctgccac ccagtccgcc ggaggactcc ggttcaggga
     7981 gcggccaact agccgagaac ctcacctatg cctggcacaa tatggacatc tttggggcgg
     8041 tcaatcagcc gggctccgga tggcggcagc tggtcaaccg gacacgcgga ctattctgca
     8101 acgagcgaca cataccggcg cccaggaaac atttgctcaa gaacggtgag tttctattcg
     8161 cagtcggctg atctgtgtga aatcttaata aagggtccaa ttaccaattt gaaactcagt
     8221 ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa
     8281 gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag ggcatccaag tatcgccatc
     8341 cgggatgcga ctgctcaatg gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc
     8401 ctatgtccag caggatgacc tctttatcgg ctccctaacg gccagggaac acctgatttt
     8461 ccaggccatg gtgcggatgc cacgacatct gacctatcgg cagcgagtgg cccgcgtgga
     8521 tcaggtgatc caggagcttt cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg
     8581 cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg gcattcgcct ccgaggcact
     8641 aaccgatccg ccgcttctga tctgcgatga gcccacctcc ggactggact catttaccgc
     8701 ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac
     8761 cattcatcag ccgtcttccg agctgtttga gctctttgac aagatccttc tgatggccga
     8821 gggcagggta gctttcttgg gcactcccag cgaagccgtc gacttctttt cctagtgagt
     8881 tcgatgtgtt tattaagggt atctagcatt acattacatc tcaactccta tccagcgtgg
     8941 gtgcccagtg tcctaccaac tacaatccgg cggactttta cgtacaggtg ttggccgttg
     9001 tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa gatatgcgac aattttgcta
     9061 ttagcaaagt agcccgggat atggagcagt tgttggccac caaaaatttg gagaagccac
     9121 tggagcagcc ggagaatggg tacacctaca aggccacctg gttcatgcag ttccgggcgg
     9181 tcctgtggcg atcctggctg tcggtgctca aggaaccact cctcgtaaaa gtgcgactta
     9241 ttcagacaac ggtgagtggt tccagtggaa acaaatgata taacgcttac aattcttgga
     9301 aacaaattcg ctagatttta gttagaattg cctgattcca cacccttctt agtttttttc
     9361 aatgagatgt atagtttata gttttgcaga aaataaataa atttcattta actcgcgaac
     9421 atgttgaaga tatgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc
     9481 atcttgattg gcctcatctt tttgggccaa caactcacgc aagtgggcgt gatgaatatc
     9541 aacggagcca tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata
     9601 aatgtaagtc ttgtttagaa tacatttgca tattaataat ttactaactt tctaatgaat
     9661 cgattcgatt taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg
     9721 actttatcgc tgtgacacat actttctggg caaaacgatt gccgaattac cgctttttct
     9781 cacagtgcca ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt
     9841 gctgcacttc ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt
     9901 cggatatcta atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc
     9961 ggttatcata ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt
    10021 atacctcaaa tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat
    10081 taaccaatgg gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg
    10141 ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct
    10201 ggactacgtg ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct
    10261 aagacttcgg gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt
    10321 ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa gctaatgtaa
    10381 ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt tccccttcga
    10441 acatccccac aagtagactt tggatttgtc ttctaaccaa aagacttaca cacctgcata
    10501 ccttacatca aaaactcgtt tatcgctaca taaaacaccg ggatatattt tttatataca
    10561 tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg tttcgtagtt
    10621 gctctttcgc tgtctcccac ccgctctccg caacacattc accttttgtt cgacgacctt
    10681 ggagcgactg tcgttagttc cgcgcgattc ggttcgctca aatggttccg agtggttcat
    10741 ttcgtctcaa tagaaattag taataaatat ttgtatgtac aatttatttg ctccaatata
    10801 tttgtatata tttccctcac agctatattt attctaattt aatattatga ctttttaagg
    10861 taattttttg tgacctgttc ggagtgatta gcgttacaat ttgaactgaa agtgacatcc
    10921 agtgtttgtt ccttgtgtag atgcatctca aaaaaatggt gggcataata gtgttgttta
    10981 tatatatcaa aaataacaac tataataata agaatacatt taatttagaa aatgcttgga
    11041 tttcactgga actagaatta attcggctgc tgctctaaac gacgcatttc gtactccaaa
    11101 gtacgaattt tttccctcaa gctcttattt tcattaaaca atgaacagga cctaacgcac
    11161 agtcacgtta ttgtttacat aaatgatttt ttttactatt caaacttact ctgtttgtgt
    11221 actcccactg gtatagcctt cttttatctt ttctggttca ggctctatca ctttactagg
    11281 tacggcatct gcgttgagtc gcctcctttt aaatgtctga ccttttgcag gtgcagcctt
    11341 ccactgcgaa tcattaaagt gggtatcaca aatttgggag ttttcaccaa ggctgcaccc
    11401 aaggctctgc tcccacaatt ttctcttaat agcacacttc ggcacgtgaa ttaattttac
    11461 tccagtcaca gctttgcagc aaaatttgca atatttcatt tttttttatt ccacgtaagg
    11521 gttaatgttt tcaaaaaaaa attcgtccgc acacaacctt tcctctcaac aagcaaacgt
    11581 gcactgaatt taagtgtata cttcggtaag cttcggctat cgacgggacc accttatgtt
    11641 atttcatcat g
//
