LOCUS       sequence-113932-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trasgenically expresses firefly luciferase in T. cruzi with neomycin
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Sanchez-Valdez FJ, Padilla A, Wang W, Orr D, Tarleton RL
  TITLE     Spontaneous dormancy protects Trypanosoma cruzi during extended drug
            exposure.
  JOURNAL   Elife. 2018 Mar 26;7. pii: 34039. doi: 10.7554/eLife.34039.
  PUBMED    29578409
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-03-26"; volume: "7. pii: 34039. doi"; pages: " 
            10.7554/eLife.34039"
COMMENT     Sequence Label: pTrex-Luc-Neo
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     218..237
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        218..236
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
      241 attggagctc ggtgcaccct gggatatttg cgcacccacc tttcccgtcg caatcggcgc
      301 cgtccgaacg cggaaatgtt ccgagaataa tacggctggg ctgcgccgcg cgttccccgc
      361 gtgaaccaac tattgtgcga tgtgcagcat cctattgtgg tgctttaccg cacaccagtt
      421 tccttgcggg gcttatttgg tgtgttggat ttttaatttt ttgatgattc ggaggaattt
      481 tttgcaattt ttgggtaccc ctaaaaaaat tttggagttt ccggggttcg atccaacttg
      541 tgctttttta accgtccatt tgtgtctagt acatcatgga gcggtattct ctccggtggc
      601 ccaggtggcg tcggaaggaa tcggtgtccc tcaggatgtt tggtttttcg tttttttttt
      661 tgcggtcttt tgtttcgcat cgccgtatcg tgtgcggcgc gaccttcttt ttggctcatt
      721 ggtcagtgag gtttataata aagtggatca tttgggtttt tggccctttg cggcgcaccc
      781 gcggaatggg ggagggattt tcatataatt gtagtgtaca cattgtgtgt gggtcgcaa
//
