LOCUS       sequence-113936-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dCas9-VP64-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-dCas9-VP64-6xHis
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcagctttc gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg
       61 tctccaatag tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc
      121 acgattttta tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact
      181 ttgtggaaca atggcatcga catcataatc acttaaacga ttaatatcta attcttggtc
      241 cacatacatg tctcttccat tttggagata atagagatag agcttttcat tttgcaattg
      301 agtattttca acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc
      361 gattcgtttc atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg
      421 tgccatttca ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc
      481 aacaactttt acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc
      541 aatatgttca tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt
      601 aaatgtcaaa ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga
      661 tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt
      721 tcgagac
//
