LOCUS       sequence-113938-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, V5 to an 
            HA-6xHIS epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pMT004
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..93
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             102..143
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      161..348
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(216..240)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(467..664)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tatagggcga attgggccca atgcattggc gccgcggccg cggtggatct ggtggatcta
       61 taacttcgta taatgtatgc tatacgaagt tatcaggtac cggtaagcct atccctaacc
      121 ctctcctcgg tctcgattct actagttaac ttctaaataa gcgaatttct tatgatttat
      181 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
      241 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
      301 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
      361 ggataacagg gtaaggatcc agattgtact gagagtgcac cataatcatc gatgaattcg
      421 agctcgtttt cgacactgga tggcggcgtt agtatcgaat cgacagcagt atagcgacca
      481 gcattcacat acgattgacg catgatatta ctttctgcgc acttaacttc gcatctgggc
      541 agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac atttgattaa aatagaacaa
      601 ctacaatata aaaaaactat acaaatgaca agttcttgaa aacaagaatc tttttattgt
      661 cagtactgat tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc
      721 gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg
      781 cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc
      841 atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat
      901 atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt
      961 ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg
     1021 ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt
     1081 caggacattg ttgg
//
