LOCUS       sequence-113939-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human CREB1 along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CREB1_2
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..109
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgcctgaaat cacttttttt caggttggac cggtgccacc atggactata aggaccacga
       61 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
      121 gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg
      181 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
      241 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
      301 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
      361 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
      421 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
      481 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
      541 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
      601 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
      661 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gac
//
