LOCUS       sequence-11394-e       13366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 13366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST>stop>shibire (ts1)
FEATURES             Location/Qualifiers
     source          1..13366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    3502..3549
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     intron          3669..3734
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3864..3884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4156..4290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5112..5159
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     5196..5212
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5196..5212
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      5222..5231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5936..5947
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7877..7893)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7878..7894)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          8004..8069
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8199..8219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8491..8625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            8643..12770
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(8652..8670)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(9121..9192,9587..9860,9935..10589,10651..10966,
                     11187..11318,11389..12003)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(12781..13335,13336..13366)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgagggtac ccggggatct tgaagttcct attccgaagt tcctattctc tagaaagtat
     3541 aggaacttca gagcgctttt gaagctagag aattcgcgca ctagtgctag aggatctttg
     3601 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     3661 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     3721 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     3781 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     3841 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     3901 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     3961 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     4021 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     4081 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4141 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4201 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4261 ctcatcaatg tatcttatca tgtctggatc tgcgtcgcgc cacttcaacg ctcgatggga
     4321 gcgtcattgg tgggcggggt aaccgtcgaa atcagtgttt acgcttccaa tcgcaacaaa
     4381 aaattcactg caacactgaa aagcatacga aaacgatgaa gattgtacga gaaaccataa
     4441 agtattttat ccacaaagac acgtatagca gaaaagccaa gttaactcgg cgataagttg
     4501 tgtacacaag aataaaatcg gccagattca gtgttgtcag aaataagaaa accccactat
     4561 gtttttcttt gccttttctt tctcccagcg atcattcatt tcgtggtgaa agaacggggt
     4621 cattgcacgg agtttcgact gcgggaaagc agagctgccg ttcacttcgt ctataattag
     4681 cgctttctat tttccccgat tcgggccgct gctgcgcttt tccgcctgct gtttgtggca
     4741 agtgtagcag caggctgtgc acgcagtgtg gcatgcactt ggctttccac cgttggtatc
     4801 gattctctgg gacgatgagt cattcctttc ggggccacag cataatcgtt gccagctcac
     4861 cgaaatggtg acttcatttc ttaactgccg tcaagcatgc gattgtacat acatacatat
     4921 ttatatatgt acatatttat gtgactatgg taggtcgata taatagcaat caacgcaagc
     4981 aaatgtgtca gtcctgctta caggaacgat tctatttagt aattttcgtt gtataaagta
     5041 attatgtatg tatgtaagcc ccataaatct gaaacaatta ggcaaaacca tgcgaggatc
     5101 cgggggatct tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
     5161 gagcgctttt gaagctgggt accgcggccg gccgctctag aactagtgga tccaaggagc
     5221 cgccgccatg gatagtttaa ttacaattgt taacaagctg caagatgcgt tcacatcgct
     5281 cggcgtccac atgcaactgg atctgccgca gatcgcggtg gtcggtggcc agtcagctgg
     5341 caagagttcc gttttggaga actttgtggg caaagatttc ttgccgcgcg gctctggtat
     5401 cgtaactcgg agaccactaa tcttgcaact gatcaatgga gttaccgaat atggcgagtt
     5461 ccttcacatt aaaggcaaga agttttccag cttcgatgag atcagaaaag aaatcgaaga
     5521 tgaaacggat cgtgtgaccg gtagtaacaa aggcatttca aacattccaa ttaatttgcg
     5581 cgtctactcg ccgcacgtac tcaatttaac cctgatcgat ttgcccggcc taacaaaggt
     5641 ggccattggc gatcaaccgg tggatattga gcagcaaatt aagcagatga tattccaatt
     5701 tattcgcaaa gagacttgtt taattttggc tgtgaccccg gccaatacgg atctggccaa
     5761 ttcggatgcc ctcaagctgg ccaaggaggt ggatccacag ggtgtgcgaa ccattggcgt
     5821 tataaccaag ctggatctga tggacgaggg caccgatgca cgcgacattc tggagaataa
     5881 gctgcttccg ctgcgacgtg gctatattgg cgtggtgaat agatcccaaa aggatatcga
     5941 ggggcgcaag gatatccacc aggcattggc cgccgaacgc aagttcttct tgagccatcc
     6001 ctcctatcgg cacatggccg accgtctcga caccccctac ttgcagcgcg tgctcaacca
     6061 acaactaacc aatcacatca gggacacgtt acctggcctg cgggataagt tgcagaagca
     6121 gatgctcact ttggagaagg aggtggagga gtttaagcac tttcagccag gcgatgctag
     6181 catcaaaaca aaagctatgc tccaaatgat tcagcagctg caatcagact ttgagcgaac
     6241 tatcgagggc agcggctcag ctttggtgaa cacaaacgag ctctcaggtg gcgccaagat
     6301 caatcgcatt ttccatgaac gtctgcgctt tgagatcgta aagatggctt gcgatgaaaa
     6361 ggaactgcga cgagagatct catttgcaat tcgaaatatc catggtattc gcgttggcct
     6421 cttcacaccc gacatggcgt tcgaggcgat tgtcaagcgg cagatagcgc tgctcaagga
     6481 gccggttatc aagtgtgtcg atctagtcgt acaggaactt tctgtggtcg tgcgcatgtg
     6541 cacagctaaa atgagtcgat acccacgtct gcgtgaggag accgaacgta ttatcacaac
     6601 acatgtgcgc caacgcgagc atagctgcaa ggaacagatc ctgctgctta ttgactttga
     6661 gctggcctat atgaacacca atcatgagga cttcattggc ttcgccaacg ctcaaaacaa
     6721 atcagagaat gccaacaaaa ctggcactcg tcaactgggc aaccaagtca ttcgcaaggg
     6781 tcacatggtc atccagaacc ttggaatcat gaaaggtgga tcgcgtccgt attggtttgt
     6841 gctgacatcc gagagtatct cctggtataa ggacgaggat gagaaggaga agaaatttat
     6901 gctgccgttg gatggtttaa aattgcgtga tattgaacag ggatttatgt caatgtctag
     6961 acgtgttaca tttgctttat ttagtcccga tggacgtaat gtttataagg actacaaaca
     7021 actggagctc tcgtgcgaga ctgtggagga tgtggagtcc tggaaagcat ccttcctgcg
     7081 agctggtgtc tatcccgaaa agcaggagac tcaggaaaac ggcgatgagg aaggacaaga
     7141 gcaaaaatcg gccagcgagg agagttccag cgatccacag ctggagcggc aggtcgagac
     7201 cattcgtaat ctggttgatt cctacatgaa gatcgttacg aagaccaccc gtgacatggt
     7261 gcccaaagca attatgatgc tgatcatcaa caatgccaag gactttatca atggcgagct
     7321 gctggcacat ctttacgcgt ccggcgatca ggcccagatg atggaggagt cggctgaatc
     7381 ggcaacacgg cgagaggaaa tgctgcgcat gtaccgtgct tgcaaggatg cattacagat
     7441 aataggtgac gtatcaatgg ccaccgtgtc gtcgccattg ccaccgccgg ttaagaacga
     7501 ttggctgccc agcggcttgg acaacccacg tctttctcct ccaagtccgg gtggcgttcg
     7561 aggaaagcca ggacctcccg cacagagctc cttgggtgga cggaacccac cattgccacc
     7621 atctacaggt cgcccagcgc cagcgattcc gaatcgccca ggaggtgggg caccaccact
     7681 acccggcggt cgtcccggag gatctcttcc cccaccaatg ctaccatcgc gtcgttaaat
     7741 tgttgtcagc gtcgagagac atctacatat gtatctaggg gatgaatctg caaaacgtag
     7801 cttgctgacg atttaaccaa accacaaaca atacccaaat tctactttca acaattgtct
     7861 aagttaacaa gcttatcgat accgtcgacc tcgagggtac ggccgcggct cgagggtacc
     7921 tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     7981 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     8041 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     8101 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     8161 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     8221 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     8281 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     8341 agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     8401 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     8461 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     8521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccacta gaaggcctaa
     8641 ttctagtatg tatgtaagtt aataaaaccc atttttgcgg aaagtagata aaaaaaacat
     8701 tttttttttt tactgcactg gatatcattg aacttatctg atcagtttta aatttacttc
     8761 gatccaaggg tatttgatgt accaggttct ttcgattacc tctcactcaa aatgacattc
     8821 cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc
     8881 gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg
     8941 ccttcagttg cttttgtcag cggtttcgtg acgaagctcc aagcggttta cgccatcaat
     9001 taaacacaaa gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg ttttttgagt
     9061 gattggggtg gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa aatcccggca
     9121 atgggccaag aggatcagga gctattaatt cgcggaggca gcaaacaccc atctgccgag
     9181 catctgaaca atgtgagtag tacatgtgca tacatcttaa gttcacttga tctataggaa
     9241 ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa
     9301 ccgcaatcgc acaaacaaat agtgacacga aacagattat tctggtagct gtgctcgcta
     9361 tataagacaa tttttaagat catatcatga tcaagacatc taaaggcatt cattttcgac
     9421 tacattcttt tttacaaaaa atataacaac cagatatttt aagctgatcc tagatgcaca
     9481 aaaaataaat aaaagtataa acctacttcg taggatactt cgttttgttc ggggttagat
     9541 gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg acagcggagc
     9601 ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg gcacgctcct
     9661 gccacccagt ccgccggagg actccggttc agggagcggc caactagccg agaacctcac
     9721 ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct ccggatggcg
     9781 gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac cggcgcccag
     9841 gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg tgtgaaatct
     9901 taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa
     9961 cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc
    10021 tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct caatggccaa
    10081 cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga tgacctcttt
    10141 atcggctccc taacggccag ggaacacctg attttccagg ccatggtgcg gatgccacga
    10201 catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga gctttcgctc
    10261 agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct gtccggcgga
    10321 gaaaggaagc gtctggcatt cgcctccgag gcactaaccg atccgccgct tctgatctgc
    10381 gatgagccca cctccggact ggactcattt accgcccaca gcgtcgtcca ggtgctgaag
    10441 aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc ttccgagctg
    10501 tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt cttgggcact
    10561 cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta agggtatcta
    10621 gcattacatt acatctcaac tcctatccag cgtgggtgcc cagtgtccta ccaactacaa
    10681 tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg
    10741 tgatcggatc gccaagatat gcgacaattt tgctattagc aaagtagccc gggatatgga
    10801 gcagttgttg gccaccaaaa atttggagaa gccactggag cagccggaga atgggtacac
    10861 ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt
    10921 gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga gtggttccag
    10981 tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga ttttagttag
    11041 aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt ttatagtttt
    11101 gcagaaaata aataaatttc atttaactcg cgaacatgtt gaagatatga atattaatga
    11161 gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc atctttttgg
    11221 gccaacaact cacgcaagtg ggcgtgatga atatcaacgg agccatcttc ctcttcctga
    11281 ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt tagaatacat
    11341 ttgcatatta ataatttact aactttctaa tgaatcgatt cgatttaggt gttcacctca
    11401 gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga cacatacttt
    11461 ctgggcaaaa cgattgccga attaccgctt tttctcacag tgccactggt cttcacggcg
    11521 attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg
    11581 ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc ctgcgccagc
    11641 tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt cctgctcttt
    11701 ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc
    11761 tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg
    11821 ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg
    11881 gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct ggccattctc
    11941 atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag
    12001 tagccgacat atatccgaaa taactgcttg tttttttttt taccattatt accatcgtgt
    12061 ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag
    12121 atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat
    12181 ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg
    12241 ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt
    12301 cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct
    12361 ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc
    12421 gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa attagtaata
    12481 aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc ctcacagcta
    12541 tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc tgttcggagt
    12601 gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg tgtagatgca
    12661 tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa
    12721 taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag aattaattcg
    12781 gctgctgctc taaacgacgc atttcgtact ccaaagtacg aattttttcc ctcaagctct
    12841 tattttcatt aaacaatgaa caggacctaa cgcacagtca cgttattgtt tacataaatg
    12901 atttttttta ctattcaaac ttactctgtt tgtgtactcc cactggtata gccttctttt
    12961 atcttttctg gttcaggctc tatcacttta ctaggtacgg catctgcgtt gagtcgcctc
    13021 cttttaaatg tctgaccttt tgcaggtgca gccttccact gcgaatcatt aaagtgggta
    13081 tcacaaattt gggagttttc accaaggctg cacccaaggc tctgctccca caattttctc
    13141 ttaatagcac acttcggcac gtgaattaat tttactccag tcacagcttt gcagcaaaat
    13201 ttgcaatatt tcattttttt ttattccacg taagggttaa tgttttcaaa aaaaaattcg
    13261 tccgcacaca acctttcctc tcaacaagca aacgtgcact gaatttaagt gtatacttcg
    13321 gtaagcttcg gctatcgacg ggaccacctt atgttatttc atcatg
//
