LOCUS       sequence-113942-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP tagged CD47 with its own long 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Berkovits BD, Mayr C
  TITLE     Alternative 3' UTRs act as scaffolds to regulate membrane protein 
            localization.
  JOURNAL   Nature. 2015 Apr 20. doi: 10.1038/nature14321.
  PUBMED    25896326
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 20. doi: 10.1038/nature14321."
COMMENT     Sequence Label: GFP_CD47_LU
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(179..181,182..184,185..697)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(224..245)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(485..504)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccat
      121 gtggcccctg gtagcggcgc tgttgctggg ctcggcgtgc tgcggatcag ctggatccat
      181 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      241 cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc gagggcgatg ccacctacgg
      301 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      361 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      421 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      481 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      541 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      601 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      661 catcaaggcg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      721 ccactaccag cagaaca
//
