LOCUS       sequence-113944-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 2xT7 to an 
            HA-6xHIS epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pMT005
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..93
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      182..369
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(237..261)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(488..685)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tatagggcga attgggccca atgcattggc gccgcggccg cggtggatct ggtggatcta
       61 taacttcgta taatgtatgc tatacgaagt tatcaggtac catggcttct atgacaggag
      121 gtcaacagat gatggcaagc atgactggtg gacagcaaat gactagttaa cttctaaata
      181 agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
      241 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
      301 tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac
      361 ctctaccggc agatccgcta gggataacag ggtaaggatc cagattgtac tgagagtgca
      421 ccataatcat cgatgaattc gagctcgttt tcgacactgg atggcggcgt tagtatcgaa
      481 tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg
      541 cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa
      601 catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga
      661 aaacaagaat ctttttattg tcagtactga ttattccttt gccctcggac gagtgctggg
      721 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
      781 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
      841 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
      901 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
      961 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     1021 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctc
//
