LOCUS       sequence-113946-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, 2xT7 to an 
            HA-6xHIS epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Terweij M, van Welsem T, van Deventer S, Verzijlbergen KF, 
            Menendez-Benito V, Ontoso D, San-Segundo P, Neefjes J, van Leeuwen F
  TITLE     Recombination-induced tag exchange (RITE) cassette series to monitor
            protein dynamics in Saccharomyces cerevisiae.
  JOURNAL   G3 (Bethesda). 2013 Aug 7;3(8):1261-72. doi: 10.1534/g3.113.006213.
  PUBMED    23708297
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-08-7- 7"; volume: "3"; 
            issue: "8"; pages: "1261-72"
COMMENT     Sequence Label: pMT005
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    26..82
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(109..126)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(127..153)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(162..195)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        290..633
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct tgcatgcctg caggtcgact ctagaggatc
       61 cccgggtacc gagctcgaat tcgcggccgc ttatgtacat taactagtgt gatggtgatg
      121 atggtgcgca tagtcaggaa catcgtatgg gtaagttcct gataacttcg tatagcatac
      181 attatacgaa gttattctag actgtgcggt atttcacacc gcatagggtt aattaaggcg
      241 cgccagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc
      301 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
      361 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
      421 ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg acctgcgagc agggaaacgc
      481 tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg
      541 ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata
      601 cagttctcac atcacatccg aacataaaca accatgggta aaaagcctga actcaccgcg
      661 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
      721 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
      781 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
      841 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
      901 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
      961 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatctt
//
