LOCUS       sequence-113948-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose inducible lambda red and anhydrotetracycline inducible 
            sgRNA expression that targets pCas9 plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Reisch CR, Prather KL
  TITLE     The no-SCAR (Scarless Cas9 Assisted Recombineering) system for 
            genome editing in Escherichia coli.
  JOURNAL   Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
  PUBMED    26463009
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-14"; volume: "5:15096. doi"; pages: " 
            10.1038/srep15096"
COMMENT     Sequence Label: pKDsgRNA-p15
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        215..290
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             311..340
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             356..373
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(429..446)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      599..645
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaacg gggtctgacg
       61 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca gctttcgcta
      121 aggatgattt ctggaattct tccctatcag tgatagagat tgacatccct atcagtgata
      181 gagatactga gcacctatcg tcttgagtcc aaccgtttta gagctagaaa tagcaagtta
      241 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgaa
      301 gcttgggccc gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca
      361 tcatcatcat cattgagttt aaacggtctc cagcttggct gttttggcgg atgagagaag
      421 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
      481 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      541 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
      601 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      661 gaactggatc cttactcgag tctagactgc aggcggatct tcacctagat ccttttaaat
      721 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgctgacagg
      781 acattatttg ccgactacct tggtgatctc gcc
//
