LOCUS       sequence-113950-       11549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  co-expresses CRE excisable human MyoD and dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Gersbach C
  TITLE     Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic 
            Cellular Differentiation in Primary Human Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV floxed hMyoD-IRES-dsRed
FEATURES             Location/Qualifiers
     source          1..11549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(374..1165,1166..1234))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     997..1016
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1235..1339)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1414..1433)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1605..1984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1986..2184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2139..2159
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2202..2382
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2429..2554
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3047..3280
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3465..3509
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3807..3924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(3962..3995)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4025..5235
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5072..5091
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    join(6284..6858,6859..6861,6862..6870)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6451..6468)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6678..6697
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6871..7548
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(7051..7071)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7269..7288
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7463..7486
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(7555..7588)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7608..8196
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7661..7681)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8079..8090)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8199..8215)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8200..8216)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8721..8901
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8927..8944)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8933..9157
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9203..9631
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9290..9309)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9500..9521
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9640..9660)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9645..9974
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9825..9960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9887..9906
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10022..10069
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10088..10462
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10592..10713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10629..10648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10683..10702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10762..10778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10762..10778)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10775..10797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10786..10802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10810..10816,10817..10834,10835..10840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10855..10876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10993..11010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11164..11549,1..203))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11244..11263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
       61 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      121 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      181 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      241 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      301 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      361 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      421 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      481 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      541 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      601 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      661 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      721 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      781 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      841 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      901 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      961 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1021 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1081 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1141 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1201 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1261 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1321 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     1381 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     1441 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     1501 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     1561 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     1621 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     1681 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     1741 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     1801 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     1861 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     1921 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     1981 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     2041 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     2101 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     2161 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     2221 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2281 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2341 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2401 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2461 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2521 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2581 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2641 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     2701 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     2761 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     2821 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     2881 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     2941 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     3001 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3061 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3121 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3181 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3241 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3301 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3361 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3421 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3481 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3541 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3601 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3661 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     3721 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     3781 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3841 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     3901 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     3961 tataacttcg tatagcatac attatacgaa gttatgttaa ttaacccgtg tcggctccag
     4021 atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
     4081 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
     4141 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
     4201 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
     4261 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
     4321 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
     4381 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg
     4441 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga
     4501 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg
     4561 agcgcacaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgta aggcgggctg
     4621 tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc
     4681 cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac
     4741 cctgacgtga agtttgtcac tgactggaga actcgggttt gtcgtctggt tgcgggggcg
     4801 gcagttatgc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gcctcgtcgt
     4861 gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt
     4921 gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg
     4981 aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg
     5041 tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg
     5101 gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg
     5161 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
     5221 gcttttttgt tagacgaagc ttgggctgca ggtcgactct agaggatccc agtgtggtgg
     5281 tacgtaggaa ttcggcccag gcggccatgg agctactgtc gccaccgctc cgcgacgtag
     5341 acctgacggc ccccgacggc tctctctgct cctttgccac aacggacgac ttctatgacg
     5401 acccgtgttt cgactccccg gacctgcgct tcttcgaaga cctggacccg cgcctgatgc
     5461 acgtgggcgc gctcctgaaa cccgaagagc actcgcactt ccccgcggcg gtgcacccgg
     5521 ccccgggcgc acgtgaggac gagcatgtgc gcgcgcccag cgggcaccac caggcgggcc
     5581 gctgcctact gtgggcctgc aaggcgtgca agcgcaagac caccaacgcc gaccgccgca
     5641 aggccgccac catgcgcgag cggcgccgcc tgagcaaagt aaatgaggcc tttgagacac
     5701 tcaagcgctg cacgtcgagc aatccaaacc agcggttgcc caaggtggag atcctgcgca
     5761 acgccatccg ctatatcgag ggcctgcagg ctctgctgcg cgaccaggac gccgcgcccc
     5821 ctggcgccgc agccgccttc tatgcgccgg gcccgctgcc cccgggccgc ggcggcgagc
     5881 actacagcgg cgactccgac gcgtccagcc cgcgctccaa ctgctccgac ggcatgatgg
     5941 actacagcgg ccccccgagc ggcgcccggc ggcggaactg ctacgaaggc gcctactaca
     6001 acgaggcgcc cagcgaaccc aggcccggga agagtgcggc ggtgtcgagc ctagactgcc
     6061 tgtccagcat cgtggagcgc atctccaccg agagccctgc ggcgcccgcc ctcctgctgg
     6121 cggacgtgcc ttctgagtcg cctccgcgca ggcaagaggc tgccgccccc agcgagggag
     6181 agagcagcgg cgaccccacc cagtcaccgg acgccgcccc gcagtgccct gcgggtgcga
     6241 accccaaccc gatataccag gtgctctgag gccaggccgg ccgcccctct ccctcccccc
     6301 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     6361 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     6421 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     6481 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     6541 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     6601 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     6661 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     6721 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     6781 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6841 gaaaaacacg atgataatat ggccacaacc atggatagca ctgagaacgt catcaagccc
     6901 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     6961 ggcgagggcg agggcaagcc ctacgagggc acccagaccg ccaagctgca ggtgaccaag
     7021 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag
     7081 gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc
     7141 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     7201 tcctccctgc aggacggcac cttcatctac cacgtgaagt tcatcggcgt gaacttcccc
     7261 tccgacggcc ccgtaatgca gaagaagact ctgggctggg agccctccac cgagcgcctg
     7321 tacccccgcg acggcgtgct gaagggcgag atccacaagg cgctgaagct gaagggcggc
     7381 ggccactacc tggtggagtt caagtcaatc tacatggcca agaagcccgt gaagctgccc
     7441 ggctactact acgtggactc caagctggac atcacctccc acaacgagga ctacaccgtg
     7501 gtggagcagt acgagcgcgc cgaggcccgc caccacctgt tccagtagga attcataact
     7561 tcgtatagca tacattatac gaagttatgg atatcaagct tatcgataat caacctctgg
     7621 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     7681 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7741 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7801 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7861 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7921 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7981 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     8041 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8101 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8161 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     8221 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     8281 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     8341 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8401 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     8461 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     8521 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     8581 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     8641 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     8701 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8881 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     8941 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     9001 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     9061 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     9121 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     9181 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9241 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9301 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9361 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9421 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9481 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9541 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9601 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     9661 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     9721 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9781 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     9841 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9901 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9961 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
    10021 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
    10081 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
    10141 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
    10201 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
    10261 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
    10321 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
    10381 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
    10441 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
    10501 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    10561 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
    10621 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10681 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
    10741 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    10801 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    10861 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    10921 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    10981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    11041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    11101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    11161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    11221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    11281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    11341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    11401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    11461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    11521 tggtaacagg attagcagag cgaggtatg
//
