LOCUS       sequence-113958-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NMD reporter mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Boehm V, Haberman N, Ottens F, Ule J, Gehring NH
  TITLE     3' UTR length and messenger ribonucleoprotein composition determine 
            endocleavage efficiencies at termination codons.
  JOURNAL   Cell Rep. 2014 Oct 23;9(2):555-68. doi: 
            10.1016/j.celrep.2014.09.012. Epub 2014 Oct 9.
  PUBMED    25310981
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.012"; journalName: "Cell Rep"; date: 
            "2014-10-23- 23"; volume: "9"; issue: "2"; pages: "555-68"
COMMENT     Sequence Label: NG0886 pCI-TPI-WT-4H
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          148..280
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     263..282
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        325..343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcagatcac tagaagcttt attgcggtag tttatcacag ttaaattgct aacgcagtca
       61 gtgcttctga cacaacagtc tcgaacttaa gctgcagtga ctctcttaag gtagccttgc
      121 agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga
      181 gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct
      241 attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat
      301 tacagctctt aaggctagag tacttaatac gactcactat aggctagcgc ctcggctcca
      361 gcaccatggc gccctccagg aagttcttcg ttgggggaaa ctggaagatg aacgggcgga
      421 agcagagtct gggggagctc atcggcactc tgaacgcggc caaggtgccg gccgacaccg
      481 gtaagccctc gccgaggagg ggtctggccg ggccggggcc ggggccgggg caggagtggc
      541 agcgccctct cccgaggccc cgaggccccg aggccggtat ccgcgcggac ctgatgcagg
      601 gctgtgggac gagggccgct ggggtccggg caggggcctc gcagccgcag ccccgtcggt
      661 gcgtcgaggg ggcagggcgg agcacatgat gccccttgga ctat
//
