LOCUS       sequence-11396-e       13768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 13768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQF-ET
FEATURES             Location/Qualifiers
     source          1..13768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          2696..2761
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2891..2911
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3183..3317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3220..3239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3274..3293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3355..3371)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3355..3371)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7854..7873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7855..7873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7883..7900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7883..7899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7892..7914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8040..8495)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8177..8198)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8389..8408
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8522..8626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8627..8695,8696..9487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9658..10246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10400..10417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10534..10555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10570..10575,10576..10593,10594..10600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10608..10624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10613..10635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10632..10648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10632..10648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10667..10687
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10669..10687
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(10776..10977,10978..11008)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(11545..11564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11664..11686
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11724..11742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11810..11914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11915..11983,11984..12775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12946..13534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gctagcttac cgaagtatac
       61 acttaaattc agtgcacgtt tgcttgttga gaggaaaggt tgtgtgcgga cgaatttttt
      121 tttgaaaaca ttaaccctta cgtgcaattc caacatgccg cctaaacgca agacactcaa
      181 tgccgctgcc gaagccaatg cccacgctga tggccatgct gatggcaatg ctgatggtca
      241 tgtcgctaac actgcagcaa gcagcaacaa cgcccgtttt gcggacttga ccaacattga
      301 cacacccggc ctcggcccta ccacgacgac gttacttgtc gagcccgctc gttcgaaacg
      361 ccagagagtc tcgagggcct gtgatcagtg tcgagctgca cgtgaaaagt gtgatggaat
      421 ccagccggct tgcttcccct gtgtgtcgca gggccggtcg tgtacctacc aggccagtcc
      481 caagaagcga ggagtccaga cgggctacat ccgcactctc gaactggctc tggcttggat
      541 gttcgagaac gttgcccgca gcgaggacgc cctccacaat cttttggtcc gtgatgctgg
      601 ccagggcagc gctctcctgg tcggcaaaga ctcgcctgct gcagaacgcc tgcatgcaag
      661 atgggcgacg agtcgagtca acaaaagcat cacccgtctt ctctcaggtc aggccgcaca
      721 agatccatct gaagacggcc aatccccgtc cgaagacata aatgtccaag atgccggggc
      781 aaagacatcc gacttccctc atgcgcctca cttgactttc tcggcgccta agtctagtac
      841 agctgagaca cgcactctac caggcccggt ccgaccacct atttcggcaa acaccctgga
      901 aaacaacctt cagccagatg gtaccgggat agggaagcta ccacccaatc attggcgcct
      961 gctggatatc tacttttcct acacgcattc ttggctccct atcctcgaga agaaagacat
     1021 gtaccaagca ttgtaccagt actctgaaca aggctcgttg cttccctctg cgaatgtcga
     1081 gtctggcgtt catgccgagc tctggagcgc gctcgccctg gcgtccttcc aggctgctgc
     1141 tactgctgca tcgagtgcta cgggtccagc ttcagctgct catggccatg acaatgccat
     1201 caatccttca cctgcagaca tatctgacac agcccgaaag ctcatacctt tggaaagcgg
     1261 gccgttccag gttcagcact gcagagcgtt gctgcttctt tgtctcgtaa gccttgggcg
     1321 ggatgattgg gagtctgctt ggttgctggt tggctttgcg gtccgcgtcc tacttgttgt
     1381 tcgcacccag ttgcctcctg atgatgaccg gccacgacca agaatgcgtg cgctgctcgt
     1441 cgcgtgcttc atcgtggata ccattgtgtc tatgagacac aacgtgccgg cccatctcaa
     1501 gccagacgac attgcggatc tgccgttacc tgaagacggt caagatcaat gggagccgtg
     1561 gacaccatgt gagggcttag gcggtgaaca caccatgctg caaatgttga ggaacccggc
     1621 atacccttta agcacattca accacctata tggcgtgacc aagctggttg ctttggagct
     1681 tctgccaaga atacgaacat cttcacagaa cgctcccttg gagttcaggt cgcggttgca
     1741 gcaggtaatc ggccacaatt ctcccttcag cgtctttgtc ctttcccagg atacagcatc
     1801 ggcttttgtg cctactgcat accttacccg taccgtttat ttatgggcag ctgccttttc
     1861 tgagcctctc aacgaacact actcgcatct tctgatcgaa actcttgatc agtatcagaa
     1921 gcggtttggt acatatgcaa tcccacctct gatcccttct cttctagact cccttcttgc
     1981 tttaaagaaa caatcacatt cttcagagcg gcatcgaagg cacttggaag agcttttccc
     2041 cgcctactcc tccatttggc ctcggggagg ccgacacagc aatactggcc tccaacccat
     2101 acgacaactt gagcttccgc cgactgcgac tgccactgca agtatcatgc cccatgtcat
     2161 ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat cggtttaatg gaataccgaa
     2221 tcctaccccc tacaatagcg atgcggccct agacgcaatt actcaaacca atgactacgg
     2281 atcagtcaat acccatggca ttctcagtac atatccacca cctgccaccc acttgaacga
     2341 ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga ccacccccgc catacgttga
     2401 tagtacaacg aaccatcctc cttaccatag taacctcgtc cccatggcca atttcggata
     2461 ttcgactgta gactatgatg cgatggtgga tgatctggca tcgatcgagt acacggatgc
     2521 ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt gtcccagggt gtaattttag
     2581 cgatatcaac acgtatggca ataggcggcc gctctagagg atctttgtga aggaacctta
     2641 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     2701 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     2761 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     2821 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     2881 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     2941 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     3001 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta
     3061 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3121 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3181 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3241 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3301 cttatcatgt ctggatcggg ccgcagcttg atatcgaatt cctgcagccc gggggatcca
     3361 ctagttctag agcggccccc cgttattctc tattcgtttt gtgactctcc ctctctgtac
     3421 tattgctctc tcactctgtc gcacagtaaa cggcactcta ttctcgttgc ttcgagagag
     3481 cgcgcctcga atgttcgcga aaagagcgcc ggagtataaa tagaggcgct tcgtcgacgg
     3541 agcgtcaatt caattcaaac aagcaaagtg aacacatcgc gaagcaagct gagcaaacaa
     3601 acaagcgcag cgaacaagct aaacaatctg caataaagtg caagttaaag tgaatcaatt
     3661 aaaagtaacc aacaaccaag taattaaact aaaaactgca actactgaaa tcaaccaaga
     3721 agtcattatt gaagacaaga agagaactct gaataggtcg atagcgtcaa tgtccgcctt
     3781 cagttgcact ttgtcagcgg tttcgtgacg aagctccaag cggtttacgc catcaattaa
     3841 acacaaagtg ctgtgccaaa actcctctcg cttcttattt ttgtttgttt tttgagtgat
     3901 tggggtggtg attggttttg ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg
     3961 ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc tgccgagcat
     4021 ctgaacaatg tgagtagtac atgtgcatac atcttaagtt cacttgatct ataggaactg
     4081 cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg
     4141 caatcgcaca aacaaatagt gacacgaaac agattattct ggtagctgtg ctcgctatat
     4201 aagacaattt ttaagatcat atcatgatca agacatctaa aggcattcat tttcgactac
     4261 attctttttt acaaaaaata taacaaccag atattttaag cttcgactct agctagatgc
     4321 acaaaaaata aataaaagta taaacctact tcgtaggata cttcgttttg ttcggggtta
     4381 gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg
     4441 acgcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc
     4501 tgccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca
     4561 cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc
     4621 ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca
     4681 ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc
     4741 ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga
     4801 acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc
     4861 ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca
     4921 acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt
     4981 tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc ggatgccacg
     5041 acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct
     5101 cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg
     5161 agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc ttctgatctg
     5221 cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc aggtgctgaa
     5281 gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct
     5341 gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac
     5401 tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct
     5461 agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca
     5521 atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc
     5581 gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc cgggatatgg
     5641 agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag aatgggtaca
     5701 cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg
     5761 tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca
     5821 gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag attttagtta
     5881 gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt
     5941 tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg aatattaatg
     6001 agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg
     6061 ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt cctcttcctg
     6121 accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt ttagaataca
     6181 tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc
     6241 agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt
     6301 tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg tcttcacggc
     6361 gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc
     6421 gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag
     6481 ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt
     6541 tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct
     6601 ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc
     6661 gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct
     6721 ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct
     6781 catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga
     6841 gtagccgaca tatatccgaa ataactgctt gttttttttt ttaccattat taccatcgtg
     6901 tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa
     6961 gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga
     7021 tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc
     7081 gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct
     7141 tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc
     7201 tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg
     7261 cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga aattagtaat
     7321 aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc cctcacagct
     7381 atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac ctgttcggag
     7441 tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt gtgtagatgc
     7501 atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat aacaactata
     7561 ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta gttctctctc
     7621 tctctctctt atctatcgct acttggttgg cgcgctctcg cgctctcttt gtgtgcgtgt
     7681 gggcagtgtg ttttttgttg ttttgcgctt tatgtgttgt attttgtgtg tttggccgaa
     7741 gtatttaaaa caaaagtgca gcggaaatag ttaataacaa aatattagtc gacgaattag
     7801 cttggctgca ggtcgacctc gaggggccgc caccgcggtg gagctccaat tcgccctata
     7861 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7921 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7981 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     8041 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     8101 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     8161 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     8221 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     8281 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     8341 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     8401 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     8461 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     8521 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8581 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8641 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8701 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8761 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8821 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8881 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8941 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9001 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9061 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9121 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9181 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9241 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9301 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9361 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9421 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9481 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9541 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9601 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9661 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9721 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9781 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     9841 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9901 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9961 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10021 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10081 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10141 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10201 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10261 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10321 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10381 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10441 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    10501 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    10561 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    10621 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
    10681 ctaaagggaa caaaagctgg gtacccgccc gggatcagat ccgcggccgg ccgcgcatag
    10741 gccactagtg gatctggatc ctctagctag agctttgcgt actcgcaaat tattaaaaat
    10801 aaaactttaa aaataatttc gtctaattaa tattatgagt taattcaaac cccacggaca
    10861 tgctaagggt taatcaacaa tcatatcgct gtctcactca gactcaatac gacactcaga
    10921 atactattcc tttcactcgc acttattgca agcatacgtt aagtggatgt ctcttgccga
    10981 cgggaccacc ttatgttatt tcatcatggt ctggccattc tcatcgtgag cttccgggtg
    11041 ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga
    11101 aataactgct tgtttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc
    11161 tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac
    11221 aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag
    11281 acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga
    11341 tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca
    11401 caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc
    11461 ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt gcggtatttc
    11521 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
    11581 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
    11641 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
    11701 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
    11761 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    11821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    11881 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    11941 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    12001 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    12061 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    12121 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
    12181 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    12241 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    12301 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    12361 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    12421 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    12481 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    12541 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    12601 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    12661 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    12721 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    12781 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    12841 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    12901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    12961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    13021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    13081 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    13141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    13201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    13261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    13321 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    13381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    13441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    13501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc ttcttcttga actcgggctc
    13561 ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg
    13621 tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa
    13681 caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
    13741 tctgccgctg gactacgtgg gtctggcc
//
