LOCUS       sequence-113963-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   De Vos D, Frederiks F, Terweij M, van Welsem T, Verzijlbergen KF, 
            Iachina E, de Graaf EL, Altelaar AF, Oudgenoeg G, Heck AJ, 
            Krijgsveld J, Bakker BM, van Leeuwen F
  TITLE     Progressive methylation of ageing histones by Dot1 functions as a 
            timer.
  JOURNAL   EMBO Rep. 2011 Sep 1;12(9):956-62. doi: 10.1038/embor.2011.131.
  PUBMED    21760613
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2011"; journalName: "EMBO Rep"; date: "2011-09-1- 1";
            volume: "12"; issue: "9"; pages: "956-62"
COMMENT     Sequence Label: pTW087
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..93
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             102..128
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             129..146
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      167..354
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(222..246)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(473..670)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tatagggcga attgggccca atgcattggc gccgcggccg cggtggatct ggtggatcta
       61 taacttcgta taatgtatgc tatacgaagt tatcaggtac ctacccatac gatgttcctg
      121 actatgcgca ccatcatcac catcacacta gttaacttct aaataagcga atttcttatg
      181 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
      241 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
      301 gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta ccggcagatc
      361 cgctagggat aacagggtaa ggatccgcat tgtactgaga gtgcaccata atcatcgatg
      421 aattcgagct cgttttcgac actggatggc ggcgttagta tcgaatcgac agcagtatag
      481 cgaccagcat tcacatacga ttgacgcatg atattacttt ctgcgcactt aacttcgcat
      541 ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac gctaacattt gattaaaata
      601 gaacaactac aatataaaaa aactatacaa atgacaagtt cttgaaaaca agaatctttt
      661 tattgtcagt actgattatt cctttgccct cggacgagtg ctggggcgtc ggtttccact
      721 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
      781 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
      841 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
      901 gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
      961 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
     1021 gtat
//
