LOCUS       sequence-113964-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human CREB1 along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CREB1_1
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        129..377
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     129..149
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     300..319
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        398..473
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        570..855
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ggttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
       61 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      121 tcacatgtga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
      181 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
      241 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
      301 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      361 ggaaaggacg aaacaccgcc acaaatcaga ttaatttgtt ttagagctag aaatagcaag
      421 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg
      481 ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa
      541 ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct
      601 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc
      661 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      721 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      781 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      841 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc at
//
