LOCUS       sequence-113965-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7
            epitope tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   De Vos D, Frederiks F, Terweij M, van Welsem T, Verzijlbergen KF, 
            Iachina E, de Graaf EL, Altelaar AF, Oudgenoeg G, Heck AJ, 
            Krijgsveld J, Bakker BM, van Leeuwen F
  TITLE     Progressive methylation of ageing histones by Dot1 functions as a 
            timer.
  JOURNAL   EMBO Rep. 2011 Sep 1;12(9):956-62. doi: 10.1038/embor.2011.131.
  PUBMED    21760613
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2011"; journalName: "EMBO Rep"; date: "2011-09-1- 1";
            volume: "12"; issue: "9"; pages: "956-62"
COMMENT     Sequence Label: pTW087
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    27..83
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(101..133)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(142..175)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        270..613
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gaattaaccc tcactaaagg gaacaaaagc ttgcatgcct gcaggtcgac tctagaggat
       61 ccccgggtac cgagctcgaa ttcgcggccg cttatgtaca acccatttgc tgtccaccag
      121 tcatgcttgc catgtcgact gataacttcg tatagcatac attatacgaa gttattctag
      181 actgtgcggt atttcacacc gcatagggtt aattaaggcg cgccagatct gtttagcttg
      241 cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca
      301 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
      361 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
      421 attacggctc ctcgctgcgg acctgcgagc agggaaacgc tcccctcaca gacgcgttga
      481 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
      541 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
      601 aacataaaca accatgggta aaaagcctga actcaccgcg acgtctgtcg agaagtttct
      661 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
      721 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
      781 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
      841 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
      901 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
      961 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     1021 attcggaccg caag
//
