LOCUS       sequence-113967-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a 
            T7-RFP tag, invariable V5 tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, 
            Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F
  TITLE     Recombination-induced tag exchange to track old and new proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 
            10.1073/pnas.0911164107. Epub 2009 Dec 14.
  PUBMED    20018668
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0911164107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-5- 5"; volume: "107"; issue: "1"; pages: "64-8"
COMMENT     Sequence Label: pKV015
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      142..339
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     terminator      complement(458..645)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     566..590
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
       61 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
      121 gagggcaaag gaataatcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca
      181 tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat
      241 attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata
      301 tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc
      361 cgccatccag tgtcgaaaac gagctcgaat tcatcgatga ttatggtgca ctctcagtac
      421 aatctggatc cttaccctgt tatccctagc ggatctgccg gtagaggtgt ggtcaataag
      481 agcgacctca tactatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa
      541 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt
      601 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagttaa
      661 ctagttttat acaattcatc cataccatgg gtaataccag cagcagtaac aaattctaac
      721 aagaccatgt ggtctctctt ttcgtttgga tctttggata aggcagattg agtggataag
      781 taatggttgt ctggtaacaa gactggacca tcaccaattg gagtattttg ttgataatgg
      841 tcagctaatt gaacagaacc atcttcaatg ttgtgtctaa ttttgaagtt aactttgata
      901 ccattctttt gtttgtcagc catgatgtaa acattgtgag agttatagtt gtattccaat
      961 ttgtgaccta aaatgttacc atcttcttta aaatcaatac cttttaattc gattctatta
     1021 actaaggtat caccttcaaa cttgacttca gctctggtct tg
//
