LOCUS       sequence-113968-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a 
            T7-RFP tag, invariable V5 tag, Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Verzijlbergen KF, Menendez-Benito V, van Welsem T, van Deventer SJ, 
            Lindstrom DL, Ovaa H, Neefjes J, Gottschling DE, van Leeuwen F
  TITLE     Recombination-induced tag exchange to track old and new proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):64-8. doi: 
            10.1073/pnas.0911164107. Epub 2009 Dec 14.
  PUBMED    20018668
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0911164107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-01-5- 5"; volume: "107"; issue: "1"; pages: "64-8"
COMMENT     Sequence Label: pKV015
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..53)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(62..95)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        190..533
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tcggaggagg ccattgtaca acccatttgc tgtccaccag tcatgcttgc catgtcgact
       61 gataacttcg tatagcatac attatacgaa gttattctag actgtgcggt atttcacacc
      121 gcatagggtt aattaaggcg cgccagatct gtttagcttg cctcgtcccc gccgggtcac
      181 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
      241 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
      301 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg
      361 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
      421 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
      481 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgggta
      541 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
      601 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
      661 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
      721 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
      781 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
      841 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
      901 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
      961 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgt
//
