LOCUS       sequence-113969-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Fatscher T, Boehm V, Weiche B, Gehring NH
  TITLE     The interaction of cytoplasmic poly(A)-binding protein with 
            eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA 
            decay.
  JOURNAL   RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014
            Aug 21.
  PUBMED    25147240
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.044933.114"; journalName: "RNA"; date: "2014-10"; 
            volume: "20"; issue: "10"; pages: "1579-92"
COMMENT     Sequence Label: pCI-MS2V5-PABPC1 MLLEmut
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          156..288
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     271..290
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        333..351
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             363..749
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
ORIGIN
        1 agtgaaccgt cagatcacta gaagctttat tgcggtagtt tatcacagtt aaattgctaa
       61 cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagtgact ctcttaaggt
      121 agccttgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      181 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      241 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      301 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctagcacca
      361 tggcttctaa ctttactcag ttcgttctcg tggacaatgg cggaactggc gacgtgactg
      421 tcgccccaag caacttcgct aacggggtcg ctgaatggat cagctctaac tcgcgttcac
      481 aggcttacaa agtaacctgt agcgttcgtc agagctctgc gcagaatcgc aaatacacca
      541 tcaaagtcga ggtgcctaaa gtggcaaccc agactgttgg tggtgtagag cttcctgtag
      601 ccgcatggcg ttcgtactta aatatggaac taaccattcc aattttcgcc acgaactccg
      661 actgcgagct tattgttaag gcaatgcaag gtctcctaaa agatggaaac ccgattccct
      721 cagcaatcgc agcaaactcc ggcatctacg gcggtggc
//
