LOCUS       sequence-113972-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NMD reporter mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Boehm V, Haberman N, Ottens F, Ule J, Gehring NH
  TITLE     3' UTR length and messenger ribonucleoprotein composition determine 
            endocleavage efficiencies at termination codons.
  JOURNAL   Cell Rep. 2014 Oct 23;9(2):555-68. doi: 
            10.1016/j.celrep.2014.09.012. Epub 2014 Oct 9.
  PUBMED    25310981
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.012"; journalName: "Cell Rep"; date: 
            "2014-10-23- 23"; volume: "9"; issue: "2"; pages: "555-68"
COMMENT     Sequence Label: NG0750 pCI-TPI-PTC48-4H
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          156..288
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     271..290
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        333..351
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agtgaaccgt cagatcacta gaagctttat tgcggtagtt tatcacagtt aaattgctaa
       61 cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagtgact ctcttaaggt
      121 agccttgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      181 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      241 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      301 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctagcgcct
      361 cggctccagc accatggcgc cctccaggaa gttcttcgtt gggggaaact ggaagatgaa
      421 cgggcggaag cagagtctgg gggagctcat cggcactctg aacgcggcca aggtgccggc
      481 cgacaccggt aagccctcgc cgaggagggg tctggccggg ccggggccgg ggccggggca
      541 ggagtggcag cgccctctcc cgaggccccg aggccccgag gccggtatcc gcgcggacct
      601 gatgcagggc tgtgggacga gggccgctgg ggtccgggca ggggcctcgc agccgcagcc
      661 ccgtcggtgc gtcgaggggg cagggcggag cacatgatgc cccttggact atggggcagg
      721 taaggacg
//
