LOCUS       sequence-11398-e       10571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pattB-QF-sv40
FEATURES             Location/Qualifiers
     source          1..10571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          7412..7477
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7607..7627
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7899..8033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7936..7955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7990..8009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8095..8164
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8383..8401)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8422..8438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8422..8438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8435..8457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8446..8462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8470..8476,8477..8494,8495..8500))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8515..8536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8653..8670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8824..9412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8904..8923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9583..10374,10375..10443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10206..10225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10444..10548)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccgggaat
     4861 tgggaattcc aacatgccgc ctaaacgcaa gacactcaat gccgctgccg aagccaatgc
     4921 ccacgctgat ggccatgctg atggcaatgc tgatggtcat gtcgctaaca ctgcagcaag
     4981 cagcaacaac gcccgttttg cggacttgac caacattgac acacccggcc tcggccctac
     5041 cacgacgacg ttacttgtcg agcccgctcg ttcgaaacgc cagagagtct cgagggcctg
     5101 tgatcagtgt cgagctgcac gtgaaaagtg tgatggaatc cagccggctt gcttcccctg
     5161 tgtgtcgcag ggccggtcgt gtacctacca ggccagtccc aagaagcgag gagtccagac
     5221 gggctacatc cgcactctcg aactggctct ggcttggatg ttcgagaacg ttgcccgcag
     5281 cgaggacgcc ctccacaatc ttttggtccg tgatgctggc cagggcagcg ctctcctggt
     5341 cggcaaagac tcgcctgctg cagaacgcct gcatgcaaga tgggcgacga gtcgagtcaa
     5401 caaaagcatc acccgtcttc tctcaggtca ggccgcacaa gatccatctg aagacggcca
     5461 atccccgtcc gaagacataa atgtccaaga tgccggggca aagacatccg acttccctca
     5521 tgcgcctcac ttgactttct cggcgcctaa gtctagtaca gctgagacac gcactctacc
     5581 aggcccggtc cgaccaccta tttcggcaaa caccctggaa aacaaccttc agccagatgg
     5641 taccgggata gggaagctac cacccaatca ttggcgcctg ctggatatct acttttccta
     5701 cacgcattct tggctcccta tcctcgagaa gaaagacatg taccaagcat tgtaccagta
     5761 ctctgaacaa ggctcgttgc ttccctctgc gaatgtcgag tctggcgttc atgccgagct
     5821 ctggagcgcg ctcgccctgg cgtccttcca ggctgctgct actgctgcat cgagtgctac
     5881 gggtccagct tcagctgctc atggccatga caatgccatc aatccttcac ctgcagacat
     5941 atctgacaca gcccgaaagc tcataccttt ggaaagcggg ccgttccagg ttcagcactg
     6001 cagagcgttg ctgcttcttt gtctcgtaag ccttgggcgg gatgattggg agtctgcttg
     6061 gttgctggtt ggctttgcgg tccgcgtcct acttgttgtt cgcacccagt tgcctcctga
     6121 tgatgaccgg ccacgaccaa gaatgcgtgc gctgctcgtc gcgtgcttca tcgtggatac
     6181 cattgtgtct atgagacaca acgtgccggc ccatctcaag ccagacgaca ttgcggatct
     6241 gccgttacct gaagacggtc aagatcaatg ggagccgtgg acaccatgtg agggcttagg
     6301 cggtgaacac accatgctgc aaatgttgag gaacccggca taccctttaa gcacattcaa
     6361 ccacctatat ggcgtgacca agctggttgc tttggagctt ctgccaagaa tacgaacatc
     6421 ttcacagaac gctcccttgg agttcaggtc gcggttgcag caggtaatcg gccacaattc
     6481 tcccttcagc gtctttgtcc tttcccagga tacagcatcg gcttttgtgc ctactgcata
     6541 ccttacccgt accgtttatt tatgggcagc tgccttttct gagcctctca acgaacacta
     6601 ctcgcatctt ctgatcgaaa ctcttgatca gtatcagaag cggtttggta catatgcaat
     6661 cccacctctg atcccttctc ttctagactc ccttcttgct ttaaagaaac aatcacattc
     6721 ttcagagcgg catcgaaggc acttggaaga gcttttcccc gcctactcct ccatttggcc
     6781 tcggggaggc cgacacagca atactggcct ccaacccata cgacaacttg agcttccgcc
     6841 gactgcgact gccactgcaa gtatcatgcc ccatgtcatg gaacagcccc tgtcaacgtc
     6901 aataaatccg gtcaatgatc ggtttaatgg aataccgaat cctaccccct acaatagcga
     6961 tgcggcccta gacgcaatta ctcaaaccaa tgactacgga tcagtcaata cccatggcat
     7021 tctcagtaca tatccaccac ctgccaccca cttgaacgag gcctcggtgg cccttgcgcc
     7081 tggaggtgct cctcccagac cacccccgcc atacgttgat agtacaacga accatcctcc
     7141 ttaccatagt aacctcgtcc ccatggccaa tttcggatat tcgactgtag actatgatgc
     7201 gatggtggat gatctggcat cgatcgagta cacggatgcg gttgacgttg atccgcagtt
     7261 catgacgaac ctcgggtttg tcccagggtg taattttagc gatatcaaca cgtatgagca
     7321 ataggacgtc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     7381 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     7441 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     7501 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     7561 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     7621 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     7681 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     7741 gctatacaag aaaattatgg aaaaatattt gatgtatagt gccttgacta gagatcataa
     7801 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     7861 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     7921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcggatctg
     8041 cggccgcggc tcgagggtac ctctagaact agtgtcgacg atgtaggtca cggtctcgaa
     8101 gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccacctcacc
     8161 catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg
     8221 gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg
     8281 acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt tctcgacggt
     8341 cacggcgggc atgtcgacac tagttctagc cagcttttgt tccctttagt gagggttaat
     8401 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8461 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8521 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8581 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9241 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9541 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9601 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9661 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9721 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9781 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9841 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9901 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9961 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10021 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10081 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10141 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10201 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10261 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10321 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10381 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10441 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10501 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10561 aaaagtgcca c
//
