LOCUS       sequence-113981-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human CEBPB along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CEBPB_2
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             53..118
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             125..145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca tggactataa
       61 ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg acgataagat
      121 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg acaagaagta
      181 cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
      241 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
      301 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa
      361 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
      421 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
      481 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga
      541 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag
      601 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg
      661 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt
      721 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg
      781 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
      841 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag
      901 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
      961 gctgagca
//
