LOCUS       sequence-113986-        3668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Parent vector used for inserting cis-regulatory elements (CREs) for 
            CRE-seq in ES cells. Uses the Pou5f1 basal promoter and dsRed gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Fiore C, Cohen BA
  TITLE     Interactions between pluripotency factors specify cis-regulation in 
            embryonic stem cells.
  JOURNAL   Genome Res. 2016 Jun;26(6):778-86. doi: 10.1101/gr.200733.115. Epub 
            2016 Apr 15.
  PUBMED    27197208
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.200733.115"; journalName: "Genome Res"; date: "2016-06";
            volume: "26"; issue: "6"; pages: "778-86"
COMMENT     Sequence Label: pGL-Pou
FEATURES             Location/Qualifiers
     source          1..3668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             501..1178
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(681..701)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     899..918
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1093..1116
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1222..1343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1233..1252)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1287..1306
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1591..1608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1762..2350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1842..1861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2550..3341,3342..3410))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3515..3563
                     /note="synthetic polyadenylation signal"
     misc_feature    3577..3668
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3617..3636
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gacctcgcta gcgtcgagga catcaagatc tggcctcggg
       61 gcccaagctt attggcacac gaacattcaa tggatgtttt aggctctcca gaggatggct
      121 gagtgggctg taaggacagg ccgagagggt gcagtgccaa caggctttgt ggtgcgatgg
      181 ggcatccgag caactggttt gtgaggtgtc cggtgaccca aggcaggggt gagaggacct
      241 tgaaggttga aaatgaaggc ctcctggggt cccgtcctaa gggttgtcct gtccagacgt
      301 ccccaacctc cgtctggaag acacaggcag atagcgctcg cctcagtttc tcccaccccc
      361 acagctctgc tcctccaccc acccaggggg cggggccaga ggtcaaggct agagggtggg
      421 attggggagg gagaggtgaa accgtcccta ggtgagccgt ctttccacca ggcccccggc
      481 tcggggtgcc caccttcccc atggatagca ctgagaacgt catcaagccc ttcatgcgct
      541 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      601 agggcaagcc ctacgagggc acccagaccg ccaagctgca ggtgaccaag ggcggccccc
      661 tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga
      721 agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg
      781 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      841 aggacggcac cttcatctac cacgtgaagt tcatcggcgt gaacttcccc tccgacggcc
      901 ccgtaatgca gaagaagact ctgggctggg agccctccac cgagcgcctg tacccccgcg
      961 acggcgtgct gaagggcgag atccacaagg cgctgaagct gaagggcggc ggccactacc
     1021 tggtggagtt caagtcaatc tacatggcca agaagcccgt gaagctgccc ggctactact
     1081 acgtggactc caagctggac atcacctccc acaacgagga ctacaccgtg gtggagcagt
     1141 acgagcgcgc cgaggcccgc caccacctgt tccagtagta attctagact cgagccgagc
     1201 tctcgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1261 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1321 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1381 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     1441 ataaggatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     1501 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     1561 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1621 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1681 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1741 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1801 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1861 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1921 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1981 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2101 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2161 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2221 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2281 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2341 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2401 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2461 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2521 cggccgcaaa tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc
     2581 tcagcgatct gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact
     2641 acgattcgtg agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt
     2701 tcaccggccc ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt
     2761 ggtcctgcta ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta
     2821 agaagttcgc cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta
     2881 tcacgctcgt cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc
     2941 acatgatcac ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc
     3001 agaagtaagt tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt
     3061 accgtcatgc catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt
     3121 tgtgagtagt gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc
     3181 gcgccacata gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa
     3241 gactcaagga tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt
     3301 tgatcttcag catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa
     3361 aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt
     3421 tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt
     3481 aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca
     3541 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     3601 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     3661 atttctct
//
