LOCUS       sequence-11400-e       10732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pattB-DSCP-QF-sv40
FEATURES             Location/Qualifiers
     source          1..10732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          7573..7638
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7768..7788
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8060..8194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8097..8116)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8151..8170
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8256..8325
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8544..8562)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8583..8599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8583..8599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8596..8618)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8607..8623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8631..8637,8638..8655,8656..8661))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8676..8697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8814..8831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8985..9573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9065..9084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9744..10535,10536..10604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10367..10386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10605..10709)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccgggaat
     4861 tgggaattcg agctcgcccg gggatcgagc gcagcggtat aaaagggcgc ggggtggctg
     4921 agagcatcag ttgtgaatga atgttcgagc cgagcagacg tgccgctgcc ttcgttaata
     4981 tcctttgaat aagccaactt tgaatcacaa gacgcatacc aaaccaattc caacatgccg
     5041 cctaaacgca agacactcaa tgccgctgcc gaagccaatg cccacgctga tggccatgct
     5101 gatggcaatg ctgatggtca tgtcgctaac actgcagcaa gcagcaacaa cgcccgtttt
     5161 gcggacttga ccaacattga cacacccggc ctcggcccta ccacgacgac gttacttgtc
     5221 gagcccgctc gttcgaaacg ccagagagtc tcgagggcct gtgatcagtg tcgagctgca
     5281 cgtgaaaagt gtgatggaat ccagccggct tgcttcccct gtgtgtcgca gggccggtcg
     5341 tgtacctacc aggccagtcc caagaagcga ggagtccaga cgggctacat ccgcactctc
     5401 gaactggctc tggcttggat gttcgagaac gttgcccgca gcgaggacgc cctccacaat
     5461 cttttggtcc gtgatgctgg ccagggcagc gctctcctgg tcggcaaaga ctcgcctgct
     5521 gcagaacgcc tgcatgcaag atgggcgacg agtcgagtca acaaaagcat cacccgtctt
     5581 ctctcaggtc aggccgcaca agatccatct gaagacggcc aatccccgtc cgaagacata
     5641 aatgtccaag atgccggggc aaagacatcc gacttccctc atgcgcctca cttgactttc
     5701 tcggcgccta agtctagtac agctgagaca cgcactctac caggcccggt ccgaccacct
     5761 atttcggcaa acaccctgga aaacaacctt cagccagatg gtaccgggat agggaagcta
     5821 ccacccaatc attggcgcct gctggatatc tacttttcct acacgcattc ttggctccct
     5881 atcctcgaga agaaagacat gtaccaagca ttgtaccagt actctgaaca aggctcgttg
     5941 cttccctctg cgaatgtcga gtctggcgtt catgccgagc tctggagcgc gctcgccctg
     6001 gcgtccttcc aggctgctgc tactgctgca tcgagtgcta cgggtccagc ttcagctgct
     6061 catggccatg acaatgccat caatccttca cctgcagaca tatctgacac agcccgaaag
     6121 ctcatacctt tggaaagcgg gccgttccag gttcagcact gcagagcgtt gctgcttctt
     6181 tgtctcgtaa gccttgggcg ggatgattgg gagtctgctt ggttgctggt tggctttgcg
     6241 gtccgcgtcc tacttgttgt tcgcacccag ttgcctcctg atgatgaccg gccacgacca
     6301 agaatgcgtg cgctgctcgt cgcgtgcttc atcgtggata ccattgtgtc tatgagacac
     6361 aacgtgccgg cccatctcaa gccagacgac attgcggatc tgccgttacc tgaagacggt
     6421 caagatcaat gggagccgtg gacaccatgt gagggcttag gcggtgaaca caccatgctg
     6481 caaatgttga ggaacccggc atacccttta agcacattca accacctata tggcgtgacc
     6541 aagctggttg ctttggagct tctgccaaga atacgaacat cttcacagaa cgctcccttg
     6601 gagttcaggt cgcggttgca gcaggtaatc ggccacaatt ctcccttcag cgtctttgtc
     6661 ctttcccagg atacagcatc ggcttttgtg cctactgcat accttacccg taccgtttat
     6721 ttatgggcag ctgccttttc tgagcctctc aacgaacact actcgcatct tctgatcgaa
     6781 actcttgatc agtatcagaa gcggtttggt acatatgcaa tcccacctct gatcccttct
     6841 cttctagact cccttcttgc tttaaagaaa caatcacatt cttcagagcg gcatcgaagg
     6901 cacttggaag agcttttccc cgcctactcc tccatttggc ctcggggagg ccgacacagc
     6961 aatactggcc tccaacccat acgacaactt gagcttccgc cgactgcgac tgccactgca
     7021 agtatcatgc cccatgtcat ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat
     7081 cggtttaatg gaataccgaa tcctaccccc tacaatagcg atgcggccct agacgcaatt
     7141 actcaaacca atgactacgg atcagtcaat acccatggca ttctcagtac atatccacca
     7201 cctgccaccc acttgaacga ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga
     7261 ccacccccgc catacgttga tagtacaacg aaccatcctc cttaccatag taacctcgtc
     7321 cccatggcca atttcggata ttcgactgta gactatgatg cgatggtgga tgatctggca
     7381 tcgatcgagt acacggatgc ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt
     7441 gtcccagggt gtaattttag cgatatcaac acgtatgagc aataggacgt ctagaggatc
     7501 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     7561 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     7621 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     7681 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     7741 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     7801 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     7861 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     7921 gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     7981 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     8041 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8101 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8161 caaactcatc aatgtatctt atcatgtctg gatcggatct gcggccgcgg ctcgagggta
     8221 cctctagaac tagtgtcgac gatgtaggtc acggtctcga agccgcggtg cgggtgccag
     8281 ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg
     8341 aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg
     8401 ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg
     8461 ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca
     8521 ctagttctag ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca
     8581 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8641 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8701 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8761 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8821 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8881 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8941 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9001 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9061 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9121 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9181 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9241 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9301 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9361 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9421 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9481 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9541 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9601 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9661 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9721 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9781 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9841 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9901 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9961 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10021 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10081 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10141 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10201 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10261 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10321 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10381 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10441 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10501 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10561 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10621 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10681 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
