LOCUS       sequence-114003-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human GABPA along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_GABPA_1
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        117..365
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     117..137
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     288..307
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        386..461
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtgagg
      121 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
      181 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
      241 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      301 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      361 acaccgaaaa ggataattga gccccgtttt agagctagaa atagcaagtt aaaataaggc
      421 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt ttagagctag
      481 aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt ctgcagacaa
      541 atggctctag aggtacccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      601 aacgaccccc gcccattgac gtcaatagta acgccaatag ggactttcca ttgacgtcaa
      661 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      721 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctgg
//
