LOCUS       sequence-114006-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3X Flag with P2A-Neo for 3' tagging of endogenous proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_Donor (EMM0021)
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     287..309
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     302..318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(514..533)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 aacggctttt tcaaaaatat ggtattgata atcctgatat gaatatattg cagtttcatt
       61 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
      121 ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgcattcgcc attcaggctg
      181 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
      241 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
      301 tgtaaaacga cggccagtga acgacggcca gtgaattgga ggctacagtc agtggagagg
      361 actttccaag atgtcttcgt ataagaagac atgatgttcg aatcagaaga actcgtcaag
      421 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
      481 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
      541 ctgatagcgg tcggccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
      601 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
      661 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
      721 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
      781 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
      841 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
      901 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
      961 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     1021 ttcattcagg gcaccggaca ggtcggtctt gacaaaaa
//
