LOCUS       sequence-114007-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology Arms and 3X Flag with P2A Neo for 3' tagging of human 
            CEBPB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_CEBPB
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..110)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(126..149)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(153..176)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(180..203)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tccaggtcca
       61 gggttctcct ccacgtctcc agcctgcttc agcaggctga agttagtagc tccgcttcct
      121 gaaaccttat cgtcgtcatc cttgtaatct cccttatcgt cgtcatcctt gtaatcgccc
      181 ttatcgtcgt catccttgta atctccagaa ccacctccac cggaacctcc tccgctcccg
      241 cagtggccgg aggaggcgag caggggctcg ggcagctgct tgaacaagtt ccgcagggtg
      301 ctgagctcgc gcgacagctg ctccaccttc ttctgcagcc gctcgttctc ggccgtgagc
      361 tccaggacct tgtgctgcgt ctccaggttg cgcatcttgg ccttgtcgcg gctcttgcgc
      421 acggcgatgt tgttgcgctc gcgccggatc ttgtactcgt cgctgtgctt gtccacggtc
      481 ttcttggcct tgctcttgac ctgcgagggc gccggcgcgg cccccgcgta gcaggcggtc
      541 gggggcgcct tggcgtcagc ggggctcggc gtgccgggcg ggctggacga ggaggacgtg
      601 gagaggctcc cgctgctgcc gctcggcacc gcctggtagc cgaggtaagc gcgcagcgcg
      661 tacgggaagc ccgccgccat gcctgcgccg ccgcccggcg ccccggcctc ctccttccgc
      721 ttgcagtccg cgggctcgaa gcccggctcc gccttgagct cggcgggcgg cggcggcggc
      781 gggggcggtg ggtgcagggg cgcgaagcag ccggggtgca gcgcgccctt ggcagccccc
      841 aggcgcccca ggctcacgta gccgtactcg gccggcttct tgcagttctt gcccc
//
