LOCUS       sequence-11401-e        5718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pBS-SK-QS
FEATURES             Location/Qualifiers
     source          1..5718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3533..3551)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3572..3588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3572..3588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3585..3607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3596..3612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3620..3626,3627..3644,3645..3650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3665..3686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3803..3820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3974..4562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4054..4073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4733..5524,5525..5593))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5356..5375
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5594..5698)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcgtta acagatcgta
      721 cccaacatga acaccatccc ggcacgccat gtcggggatg tcgccgcccg cgatcctcta
      781 cctctaccgc acatatcatc ctccgtcgcc agcggcatga agcgttcctt cgcaaccatg
      841 gccatgctct acaacgacac tggcaacagc aacgatgtcg gtgcccatgc caggcgacca
      901 ccacgaaccc tctccaatag tcggagcact tccgcccaca gagtacctct aggctcttgg
      961 tcggcgccca attccccgcc ccgccgtgcg ctgccgcatc atcccatcac cgccagcttc
     1021 gatcccgatg cttccattgt tatcgccggc attcgtggcg ctggcaagtc tacgctggcc
     1081 atcatggcat ctaccgccat gaagcgcaag atagtcgacc tggaatccga gttccatcat
     1141 cttactggct tgtctagttc cagctacaag aagacacacg gcccggtcga ctatgggagg
     1201 cgccagatcg ccatcttgca gaacatcttg aatctgcaca ggacccgcgc cattctcgtc
     1261 tgctcttggc tggagcggga tgtgcaggcc atgttgcagg atttcagcgt gtctaatcct
     1321 gtcatttacg ttctgcgcga tgccaaagct atcgaggccc atctgaaggg atacgacaag
     1381 tccaaagttg gcaccctcct tgatgccacc agtactgtcc ttcgccgctg cacccgtttc
     1441 gagttcttca acgtctccga agagaacctg gacacccact ccgcttcaac atcaccacct
     1501 gctgttccgg accagcggca taccgcgccg tatctaacgc ttaaacgagc cgagcgccac
     1561 ttcctcaaat ttctctcctt gattctaccc aaggggacca taccttttgt cgagtccgcc
     1621 tttcccctgg cttccgtccc cgttgaacag cgccgcttta cctacgccct cgccttgcct
     1681 gtatctgcct tgctcgacaa aggcgtcgat atccaagagc ttgatgtcgg tgtagacgca
     1741 atcgagatca ttgtagacga tcttgcaacg agcgaatccg gcccaacgag ccccttgggt
     1801 cttgcgcccc accgagcgag cgagatcagt cgtgttgtag gcgaaatcag gagggacaca
     1861 gtgatcccca tcattctgca cgtggtcttt ccagaaagag cgctatatga agaagctctg
     1921 ctcgcgcttt acatgactta cctgaaccat gccttaaggc ttgcgccaga ttatcttacg
     1981 gtcgatctgg ggctcgattc tggcttgctt gggcaactaa ccaccgttca aggaaccacc
     2041 aaggtcatcg gcaataaaca acttgcagag gtcaattcgc cgcgctgggg ggatccatct
     2101 tggttacaag cctatgaaaa ggcccagaat acaggatgcg acttggtaag gttgaccaga
     2161 ccggcttcaa atccccggga caacacagac attcggcagt tccacgttgc tgtagaggcc
     2221 gtcgggggtc caaggctccc atttattgct tacaacacag gacgcctagg tcggacatcg
     2281 atgtgtttta acgagatcct gactccagtt acaccagtgc ctaccaagga ggatgcaatc
     2341 gggctccgca atccagccca tcgctatctc cagcctccgc tcacggctct ggaagcaaca
     2401 caggctctct actcggcatt tgtccacgac ccaatgaagc tgtatgtctt tggcgcaaat
     2461 gtgggatata gcttgtcccc agccatgcac aatgccgcac tcaaggcctg tggcattcca
     2521 caccattaca agcccctttc cacagcaaac atcgggactt tgcgcgaggt tatcagcgat
     2581 ccgcagtttg ctggagcctc ggtcggcctg ccgtttaagg tggaaatcat cagcctcaca
     2641 cactcgctga gccggcacgc gaaagccatc ggagccgtca acaccttgat tccggtacga
     2701 caccttaccg cggacggtgg aataccggac gaggtgtcca tgttcaacaa tatcagccaa
     2761 gccggcgctg tcagagctct ctacggcgag aacacggatt ggattggtat ccgagcctgc
     2821 cttcgccgcg gtttatcgcc cgccaatgcc gtgagatcaa caagcactgg tcttgtcatc
     2881 ggcgctggcg gaatggctag ggcagctgtc tatgccatgc ttcaactggg agtcaagaag
     2941 attttgatct ttaaccgaac atttgctaat gccgagaagc tggttctaca cttcgagaac
     3001 ctgttggtca gagacgcatt gcctctgttg agcacagggc caagatccca cgacaacacc
     3061 tgttttcaca tcattcgatc tcgagacgat ccgctcccag aaaacttcaa aaacccgacc
     3121 atgatcgttt cctgcatacc gacacacaca gtggacaaca cccctgaccc tgaatttact
     3181 gtgcctttgc actggctcga caaccccact ggcggcattg tactagaact cgactacaaa
     3241 tgtctcacat cacccttgct cgaacaaaca cgacgcgagg ctcacagagg ctgggtcgca
     3301 atggatggac ttgacctctt gccagaacaa gggtttgccc aatttgaact gttcaccggg
     3361 cggcgagcac ctcgtcgctt gatgaggcgc gaggttttgc gagcataccc agatgatcaa
     3421 gcaaaatctc ataccgcgca gttacagcct cgcctcaacg gaattgcaac gcaaatatct
     3481 tgagcggccc tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     3541 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4381 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5581 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5701 catttccccg aaaagtgc
//
